Testing reagent for serum amyloid protein A and preparation method of testing reagent

A serum starch and protein technology, which is applied in biological testing, color/spectral property measurement, material inspection products, etc., can solve the problems of cumbersome operation steps, low sensitivity and low degree of automation.

Inactive Publication Date: 2016-10-12
浙江达美生物技术有限公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The colloidal gold method is easy to operate, fast, and the result judgment is intuitive, but the detection sensitivity is low; the detection sensitivity of the ELISA met

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Testing reagent for serum amyloid protein A and preparation method of testing reagent
  • Testing reagent for serum amyloid protein A and preparation method of testing reagent
  • Testing reagent for serum amyloid protein A and preparation method of testing reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] A kind of assay reagent of serum amyloid A (SAA) of the present embodiment comprises R1 reagent, R2 reagent and serum amyloid A standard substance, and described R1 reagent main component is Tris (trishydroxymethylaminomethane), NaN 3 ; The main components of the R2 reagent are latex particles coated with anti-human serum amyloid A antibody, NaN 3 The main component of described serum amyloid A standard item is serum amyloid A, bovine serum albumin (BSA), phosphate buffer saline, NaN 3 .

[0020] Have the preparation method of above-mentioned characteristic serum amyloid A (SAA) measuring reagent, comprise the steps:

[0021] (1) Preparation of R1 reagent:

[0022] ① Add 2.0L Tris (trishydroxymethylaminomethane) buffer solution into an appropriate container;

[0023] ② Turn on the electric stirrer, and the stirring speed is 450 rpm;

[0024] ③ Add 2.375g NaN 3 Add to the above solution and stir for ten minutes until completely dissolved;

[0025] ④ Dilute to 2.5L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a testing reagent for serum amyloid protein A and a preparation method of the testing reagent and belongs to the technical field of testing of biochemical means. The testing reagent comprises a reagent R1, a reagent R2 and a serum amyloid protein A standard substance, wherein main ingredients of the reagent R1 are trihydroxymethyl aminomethane and NaN3; main ingredients of the reagent R2 are latex granules enveloping antibodies resisting to human serum amyloid protein A as well as NaN3; main ingredients of the serum amyloid protein A standard substance are serum amyloid protein A, bovine serum albumin, a phophate buffer solution and NaN3. The testing reagent is used for testing serum amyloid protein A and has the advantages of fastness, sensitivity, good accuracy, good stability, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention relates to a reagent for measuring serum amyloid A and a preparation method thereof, belonging to the technical field of biochemical means testing. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is a polymorphic protein family encoded by multiple genes, the precursor of tissue amyloid A, and belongs to the acute phase response protein. In the acute phase of inflammation or infection, it rises rapidly within 48-72 hours, and decreases rapidly during the recovery phase of the disease. At present, elevated serum SAA has been detected in bacterial, viral infection, atherosclerosis, coronary heart disease, acute transplant rejection, tumor and other diseases. For certain diseases, such as viral infection, transplant rejection, coronary heart disease, etc., the sensitivity of SAA is higher than that of CRP, which can provide better reference value for clinical practice. As a new detection indicator, SAA is receiving more...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N21/31
CPCG01N33/68G01N21/31
Inventor 方朝君
Owner 浙江达美生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap