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Fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules

A technology of Shouwu Yizhi capsules and fingerprints, which is applied in the direction of color/spectral characteristic measurement, measuring devices, instruments, etc., can solve the problems of not being able to comprehensively, objectively and scientifically evaluate the quality of Shouwu Yizhi capsules, and avoid errors , Good stability, monitor the effect of stability

Active Publication Date: 2016-10-26
SHANDONG ACAD OF CHINESE MEDICINE +1
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Problems solved by technology

[0005] The present invention aims at the problem that the prior art cannot comprehensively, objectively and scientifically evaluate the quality of Shouwu Yizhi Capsules, and proposes a HPLC fingerprint determination method

Method used

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  • Fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules
  • Fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules
  • Fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules

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Embodiment 1

[0034] A fingerprint assay method for Shouwu Yizhi capsules, comprising the following steps:

[0035] (1) Preparation of reference solution: Weigh emodin, emodin methyl ether, 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside, salvianolic acid B , An appropriate amount of tanshinone Ⅱ A reference substance, salvianolic acid B was dissolved in 75% methanol, and the rest of the reference substance was dissolved in methanol to make reference solution respectively, in which the concentration of emodin reference substance solution was 10.26 μg / mL, emodin methyl ether reference substance Solution concentration is 9.68µg / mL, 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside reference substance solution concentration is 64.08µg / mL, salvianolic acid B reference substance solution Concentration of 0.15mg / mL, tanshinone Ⅱ A reference substance solution concentration of 20μg / mL, 2 , 3 , 5 , 4'-tetrahydroxystilbene-2-O-β-D-glucoside, tanshinone ⅡA reference substance solution avoid Ligh...

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Abstract

The invention relates to a quality control method of polygonum multiflorum-Alpinia oxyphylla capsules, in particular to a fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules, and belongs to the technical field of medicine analysis. The fingerprint spectrum detection method includes the steps of firstly, preparing a control solution; secondly, preparing an internal standard solution; thirdly, preparing a test solution; fourthly, using high performance liquid chromatography to perform analysis and detection. The fingerprint spectrum detection method has the advantages that an internal standard peak is innovatively quantitatively added into the complex fingerprint spectrum of traditional Chinese medicine preparations so as to calibrate the chromatographic peaks in the preparations, and the method is accurate, simple, high in sensitivity and good in reproducibility; the method can comprehensively monitor the quality of semi-finished and finished products and the stability of production process.

Description

technical field [0001] The invention relates to a quality control method of Shouwu Yizhi capsules, in particular to a fingerprint determination method of Shouwu Yizhi capsules, and belongs to the technical field of drug analysis. Background technique [0002] Shouwu Yizhi Capsule is a traditional Chinese medicine capsule made by combining 10 kinds of traditional Chinese medicines and extracts such as Polygonum multiflorum, Yizhi, and Astragalus, and through a specific processing and extraction process. Shouwu Yizhi Capsules are combined with all prescriptions, nourishing but not stagnant. The combination of various medicines can nourish, warm and nourish the essence and blood, and pass through the blood vessels. Blood stasis blocking collateral type vascular dementia can significantly improve the cognitive ability, memory ability and daily life ability of patients, effectively improve the clinical symptoms of traditional Chinese medicine, and improve the quality of life of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02G01N30/06G01N30/14G01N21/33
CPCG01N21/33G01N30/02G01N30/06G01N30/14G01N30/88G01N2030/027G01N2030/062G01N2030/146G01N2030/8809
Inventor 丁晓彦赵渤年刘青刘丽李鹏贾元印
Owner SHANDONG ACAD OF CHINESE MEDICINE
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