External and internal standard fingerprint spectrum measuring method of honeysuckle flower and radix scutellariae particle

Abstract

The invention relates to a quality control method of a honeysuckle flower and radix scutellariae particle, in particular to a fingerprint spectrum measuring method of the honeysuckle flower and radixscutellariae particle, and belongs to the technical field of medicine analysis. The fingerprint spectrum measuring method comprises the following steps: (1) preparation of a reference product solution; (2) preparation of an internal standard solution; (3) preparation of a test product solution; (4) analyzing and measuring by high performance liquid chromatography-mass spectrography. The fingerprint spectrum measuring method has the advantages that the high performance liquid chromatography-mass spectrography is adopted to precisely measure chemical components at a common peak of the honeysuckle flower and radix scutellariae particle; the operation is simple and accurate, the sensitivity is high, and the repeatability is high; the qualities of crude drug materials, semi-finished products and finished products can be more comprehensively monitored, and the stability of the production technology is monitored.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for determining the fingerprint of silver yellow granules. Background technique [0002] Yinhuang granule is a compound preparation composed of honeysuckle extract and scutellaria baicalensis extract. It has the functions of clearing heat, detoxifying, and reducing inflammation. It is widely used in the treatment of upper respiratory tract infection, acute tonsillitis, pharyngitis, mumps, ophthalmic diseases, and burn infection. and other diseases. At present, there are 102 enterprises that have obtained the approval number of this variety from the State Food and Drug Administration, and there are many manufacturers. In addition, differences in raw materials and production processes will affect product quality. [0003] Chinese medicine fingerprints use various advanced analysis methods (spectrum, spectrum, chromatography, etc.) to mark the characteristics of Chin...

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Problems solved by technology

[0004] In the paper "HPLC Characteristic Spectrum Analysis of Yinhuang Granules" in "Journal of Pharmaceutical Analysis", Volume 29, No. 8, 2009, a RP-HPLC fingerprint determination method for Yinhuang Granules was established, and the mobile phase was acetonitrile-0.1% phosphoric acid solution, gradient elution, the flow rate is 1.0mL / min, and the detection time is 35min. Using the peak of chlorogenic acid contained in itself as the reference peak, a total of 12 common peaks are determined as the mathematical expression of the fingerprint of this variety. This document adopts its own The composition is used as a reference, which cannot reflect the differences of different batches of samples, and cannot quantitatively express the chemical information of samples

In the paper "Ultra-high Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry for the Determination of Chemical and Main Components in Yinhuang Granules" in "Analytical Laboratory", Volume 34, No. 7, 2015, the ultra-high performance liquid Phase chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) is a qualitative and quantitative analysis method for the determination of active ingredients in Yinhuang granules, using 0.5% formic acid aqueous solution-acetonitrile as the mobile phase, gradient elution, and a flow rate of 0.4 mL / min , the detection time is 25 min, and the 12 known components in the chromatogram are quantitatively detected. This document only quantitatively expresses the known components, which cannot meet the needs of our spectrum-efficiency correlation mathematical model to quantify all components of the fingerprint express basic requirements

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