Diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid and use method thereof

A diagnostic kit and rapid determination technology, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effects of avoiding increased costs, accurate and reliable results, and shortening diagnostic time

Inactive Publication Date: 2016-11-09
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of diagnostic dosimetry to detect the sensitivity of melon aphid to pesticides has not been reported.

Method used

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  • Diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid and use method thereof
  • Diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid and use method thereof
  • Diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Such as figure 1 As shown, the diagnostic kit for rapidly measuring the sensitivity of aphids to acetamiprid of the present invention includes a finger tube 1, a drug film 2 and a filler, and the finger tube 1 is a glass tube with one end closed, and the The drug film 2 is an acetamiprid drug film evenly attached to the inner surface of the finger tube 1 , and the filler is detachably filled in the open end of the finger tube 1 . The finger tube 1 is a cylindrical tube, the length of the finger tube 1 is 8-10 cm, and the inner diameter is 2.5-3 cm. The drug film 2 is uniformly applied to the inner wall of the finger tube 1 by rolling acetamiprid acetone solution, and the content of acetamiprid in the drug film 2 is 0.078 μg / cm 2 .

[0025] Preferably, the filler is a tampon, the tampon is a sterilized absorbent cotton ball, and the size of the tampon matches the size of the mouth of the finger tube 1 .

[0026] The method for using the diagnostic kit for quickly dete...

Embodiment 2

[0045] Dissolve acetamiprid original drug with acetone, dilute it into 7.20mg / L acetamiprid acetone solution, take 1ml of the drug solution and add it into a finger-shaped tube with a length of 9cm and an inner diameter of 3cm, and roll it fully so that the drug solution is evenly attached to the A drug film is formed on the inner wall of the finger-shaped tube. After the acetone in the tube is completely volatilized, a cotton plug is plugged into the tube mouth, and the rapid diagnostic kit is completed.

[0046] The prepared diagnostic kits were taken to cucumber fields in Malianwa, Beijing, and Langfang, Hebei, where the melon aphids occurred seriously. In the fields, the leaves with a large number of aphids were picked, and the aphids were gently swept into the diagnostic kits with a small brush. 30 heads per tube, 3 tubes for each population, and the mortality of the melon aphid was checked after 3 hours. The diagnostic results were: the mortality rate of the two populati...

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Abstract

The invention relates to a diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid. The diagnostic kit comprises a finger-type pipe, an agent film and a filler. The finger-type pipe is a glass pipe with one enclosed end. The agent film is an acetamiprid agent film uniformly adhering to the inner surface of the finger-type pipe. The opening end of the finger-type pipe is detachably filled with the filler. The diagnostic kit is used for fast diagnosis of field aphis gossypii glover sensitivity to acetamiprid, shortens diagnosis time, produces accurate and reliable results and prevents adverse results such as cost increasing, prevention and treatment failure and environment pollution caused by use of acetamiprid in a resistant population.

Description

technical field [0001] The invention relates to the technical field of rapid detection of pest sensitivity, in particular to a diagnostic kit and a use method for rapidly measuring the sensitivity of the melon aphid to acetamiprid. Background technique [0002] Melon aphid (Aphis gossypii Glover), also known as cotton aphid, mainly damages melon vegetables, and sucks plant juice on the back of leaves and tender stems with adults and nymphs. In my country, except for Tibet, which has not been reported, it has occurred in various places. After the young leaves and growth points of melon seedlings are damaged, the leaves will shrink, and the melon seedlings will wilt or even die. The old leaves are damaged and fall early, shortening the fruiting period and reducing production. Hosts include cucumbers, pumpkins, zucchini, watermelons, beans, eggplants, spinach, green onions, onions and other vegetables, as well as crops such as cotton, tobacco, okra, melons, cantaloupe, edible...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085G01N2333/43552
Inventor 张燕宁蒋红云张兰冯磊毛连纲
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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