Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Article and method for detecting aerobic bacteria

A composition and substrate sheet technology, applied in the detection of aerobic bacteria in samples, devices for culturing and detecting microorganisms, and in the field of culturing and detecting microorganisms in samples, which can solve the problems of inconvenience, time-consuming, and inconvenience to end users.

Inactive Publication Date: 2016-11-09
3M INNOVATIVE PROPERTIES CO
View PDF14 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, the use of conventional curing agents is often inconvenient for the end user
For example, when using the standard "plate count" or "pour plate" method to determine the number of microorganisms in a liquid sample (eg, water or milk), the use of conventional agar media is particularly inconvenient and time-consuming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Article and method for detecting aerobic bacteria
  • Article and method for detecting aerobic bacteria
  • Article and method for detecting aerobic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach A

[0121] Embodiment A is a device for cultivating and detecting microorganisms, the device comprising:

[0122] A self-supporting substrate sheet having a first major surface and a second major surface;

[0123] a cover sheet attached to the base sheet;

[0124] a sample receiving area disposed between the base sheet and the cover sheet;

[0125] A first layer comprising a substantially dry, cold water soluble first hydrogel-forming composition attached to the first major surface of the substrate sheet; and

[0126] Multiple indicators, said multiple indicators include:

[0127] Three indicators for the detection of different glycosidase enzyme active components;

[0128] An indicator for the detection of the active ingredient of the alkyl esterase enzyme;

[0129] Indicators for the detection of phosphatase enzyme active components;

[0130] Contains tetrazole Redox indicators for dyes;

[0131] wherein each of the plurality of indicators comprises a detectable reporter...

Embodiment approach H

[0139] Embodiment H is the device of any one of the preceding embodiments, wherein the cover sheet comprises a first major surface, wherein the first major surface of the cover sheet faces the first major surface of the base sheet, wherein the culture device further comprises:

[0140] comprising a third layer of the second adhesive composition, wherein the third layer is attached to a portion of the cover sheet; and

[0141] A fourth layer comprising a substantially dry, cold water soluble second hydrogel-forming composition, wherein the fourth layer is attached to the third layer.

[0142] Embodiment 1 is the device of any one of the preceding embodiments, wherein at least one of the plurality of indicators is disposed in the first adhesive composition, the second adhesive composition, the first hydrogel-forming combination and / or the second hydrogel-forming composition.

[0143] Embodiment J is the device of embodiment I, wherein at least three of the plurality of indicato...

Embodiment approach W

[0156] Embodiment W is the device of any one of the preceding embodiments, wherein the first hydrogel-forming composition or the second hydrogel-forming composition comprises a substantially dry agglomerated powder.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Application No. 61 / 949,631, filed March 7, 2014, the disclosure of which is incorporated herein by reference in its entirety. Background technique [0003] Aerobic plate count (APC) is used as an indicator of the total microbial load in a sample (eg, a food sample). Typically, APC is performed using the pour plate method, surface coating method, spiral plate count method, or membrane filter method; all of these methods typically utilize agar media to grow and enumerate microorganisms. [0004] Media for growing bacteria are usually prepared by dispersing a solidifying agent in an aqueous solution containing nutrients and other ingredients necessary for the growth of a particular microorganism. Unfortunately, the use of conventional curing agents is often inconvenient for the end user. For example, the use of conventional agar media is particularly inconvenient ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/04
CPCC12M41/36C12Q1/04C12Q2334/52C12Q1/34C12Q1/42C12Q1/44
Inventor 塞拉嘉·常德拉帕蒂特拉·M·诺德比
Owner 3M INNOVATIVE PROPERTIES CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com