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Kit for detecting polymorphism of UGT1A1 gene

A gene polymorphism and kit technology, applied in the biological field, can solve the problems of wrong judgment, high cost, etc., and achieve the effects of accurate sequencing, less genetic material, and short analysis time

Inactive Publication Date: 2016-12-14
CHANGCHUN HENGXIAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This product has a certain degree of subjectivity in the interpretation of results, and there is a possibility of misjudgment and it is expensive

Method used

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  • Kit for detecting polymorphism of UGT1A1 gene
  • Kit for detecting polymorphism of UGT1A1 gene
  • Kit for detecting polymorphism of UGT1A1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: detection kit and its use

[0049] (1) Prepare a kit including the following components: 1 tube of primer mix (50μl / tube), 1 tube of Taq enzyme (7μl / tube), 1 tube of sequencing primer (45ul / tube), 1 tube of buffer solution (125ul / tube ), dNTP1 tube (100ul / tube).

[0050] (2) Specimen collection, transportation and storage:

[0051] 1. Specimen collection: The specimen is blood. 2ml of venous blood was taken routinely, and treated with EDTA anticoagulant.

[0052] 2. Storage: It can be detected immediately, stored at 4°C for one week, and stored at -20°C for up to one year.

[0053] 3. Transportation: Specimens should be transported using 0°C ice cubes.

[0054] (3) Detection steps and result analysis:

[0055] 1. DNA extraction

[0056] The Qiagen DNA extraction kit is recommended.

[0057] DNA extraction from peripheral whole blood: centrifuge 1.5ml of peripheral whole blood at 1000g for 10 minutes at 4°C, and discard the supernatant. Add 200μl ic...

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Abstract

The invention relates to a kit for detecting the polymorphism of a UGT1A1 gene, and belongs to the field of biotechnology. The aim of the invention is to develop a kit for detecting the polymorphism of UGT1A1 gene by quick and accurate analysis of UGT1A1 gene polymorphism by the gene polymorphic line through a pyrosequencing platform. The kit comprises primers, Taq enzyme, a buffer, a d-NTP, a plurality of capped sealed reagent bottles or tubes, and packing boxes for separation and concentrated package of the reagent bottles or tubes. The primer mixture comprises a forward primer, a reverse primer and sequencing primers. The amplification range of the kit can cover all normal people and patients with irinotecan medication. The kit has the characteristics of short analysis time, high throughput and less genetic material.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] Since the Food and Drug Administration (FDA) approved irinotecan (CPT-11)-line therapy for metastatic colorectal cancer in 1998, CPT-11-related toxicities, namely delayed diarrhea and neutropenia The reduction of cells has caused widespread concern of clinicians. [0003] Irinotecan (CPT-11) is a semi-synthetic derivative of camptothecin. Its main metabolic site is in the liver. After intravenous injection, it is hydrolyzed by carboxylesterases (CES) in vivo and converted into its active metabolite. 7-Ethyl-10-hydroxycamptothecin (7-ethyl-10-hydroxy-camptothecin, SN-38), inhibits DNA topoisomerase Ⅰ and exerts cytotoxic effect. It has good curative effect on colorectal cancer, gastric cancer and small cell lung cancer. [0004] Studies have shown that irinotecan can improve the chemotherapy efficacy, progression-free survival time (progression-free survival, PFS) and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/106C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 蔡燕宁杜培革于顺安利萍王敖雪赵雪隋宝珍王玉华关恒
Owner CHANGCHUN HENGXIAO BIOTECH CO LTD
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