Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Serum-free medium for dendritic cells (DCs)

A technology of serum-free medium and basal medium, which is applied in the direction of cell culture active agent, culture process, tissue culture, etc. It can solve the problems of unstable medium components, achieve good functional consistency, stable components, and increase the value-added multiple Effect

Inactive Publication Date: 2016-12-21
英普乐孚生物技术(上海)有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above-mentioned problems and / or other problems in the related art, the present invention provides a serum-free medium for DC cells with more stable components, which improves the instability of the medium components caused by the complex composition of albumin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum-free medium for dendritic cells (DCs)
  • Serum-free medium for dendritic cells (DCs)
  • Serum-free medium for dendritic cells (DCs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～4

[0027] The preparation process of DC cell serum-free medium in Examples 1-4 is as follows:

[0028] Step 1: Obtain fatty acid-free albumin;

Embodiment 1

[0029] Embodiment 1 Get bovine serum albumin (the A1933 product purchased from SIGMA company) 1.5mmol, add water for injection and be made into 2000ml solution, add 50g gac, mix; Adopt the hydrochloric acid solution of 2mol / L to adjust the pH value to pH3.0; Place it in a water bath at 56°C for 30 minutes or place it overnight at 4°C; centrifuge (4000 rpm) to remove the activated carbon; then use 2mol / L NaOH solution to adjust the pH value to pH7.0; finally filter and sterilize to obtain the product of Example 1 fatty acid-free albumin solution;

Embodiment 2

[0030] Example 2 Take human serum albumin (purchased from Grifols (Grifols) company's human serum albumin (the product specification is 20% 50ml*10g / bottle, wherein the content of sodium octanoate is not more than 5mol / mol albumin)) 1.5 mmol, the specific operation process is the same as in Example 1, and the fatty acid-free albumin solution of Example 2 is obtained;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a serum-free medium for dendritic cells (DCs), and a preparation method of the serum-free medium. The preparation method comprises the steps of loading fatty acid onto fatty acid-free albumin, and then adding the obtained albumin solution into a basic medium. The obtained serum-free medium for the DCs is more stable in components and better in functional consistency, and difference cannot be caused by different species origins or processing batches of the albumin.

Description

technical field [0001] The invention relates to a DC cell serum-free medium, which belongs to the technical field of cell culture. Background technique [0002] Serum-free medium refers to a synthetic medium that can maintain the growth and reproduction of cells in vitro for a long time without adding serum, and can be used to cultivate immune cells, such as lymphocytes (for example, T lymphocytes, B cells, NK cells, etc.) , dendritic cells (also known as DC cells), monocytes / macrophages, etc. [0003] Generally speaking, the nutrients required for the survival and expansion of immune cells in vitro include: albumin, transferrin, insulin, amino acids, vitamins, sugars, lipids, inorganic salts, growth factors, etc. Among these components, albumin has the greatest impact on cell growth and survival, and is also the component with the most complex function and composition. In serum-free medium, the function of albumin is mainly determined by the fatty acids and trace elements...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784
CPCC12N5/0639C12N2500/24C12N2500/30C12N2500/46C12N2500/90C12N2501/22C12N2501/2304C12N2501/33C12N2501/998C12N2501/999
Inventor 武宁谢志明
Owner 英普乐孚生物技术(上海)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com