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Application of cell nucleus size and shape measuring method in reproductive toxicity evaluation

A measurement method and cell nucleus technology, which is applied in the application field of cell nucleus size morphometric measurement in reproductive toxicity evaluation, can solve the problems of time-consuming, huge number of spermatocytes, and large labor and material resources.

Inactive Publication Date: 2017-01-04
INST OF SCI & TECH GUANGDONG UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0010] Conventional spermatocyte classification and identification technology mainly uses nuclear staining to observe the shape of nuclear chromatin. This method has the technical advantages of accurate detection, but the disadvantages are also quite obvious: the number of spermatocytes is quite large, and manual classification is required. It takes a lot of manpower and material resources to judge; it takes a long time and technicians need a certain level of knowledge and specific training operations

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  • Application of cell nucleus size and shape measuring method in reproductive toxicity evaluation
  • Application of cell nucleus size and shape measuring method in reproductive toxicity evaluation
  • Application of cell nucleus size and shape measuring method in reproductive toxicity evaluation

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Embodiment Construction

[0021] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. The specific conditions are not indicated in the examples, which are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0022] 1 Experimental animals: 60 Beagle dogs, with a weight range of 8-10kg, feeding conditions: raised in a normal environment animal room, in line with the requirements of the national standard GB14925-2010.

[0023] 2. The dogs were euthanized. Before the experiment, 30mg / ml pentobarbital sodium solution was injected intramuscularly according to the body weight of 3...

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Abstract

The invention relates to a cell nucleus size and shape measuring method. Testicular tissue slices of male animals are analyzed by using a microscopic image analysis system, so that leptotene stage spermatocytes, pachytene stage spermatocytes and spermatids are quickly and automatically classified. The method has huge advantages of quickness, accuracy and human and material resource saving, and provides an important evaluation basis for reproductive system toxicity evaluation of dogs and other male animals in non-clinical safety evaluation.

Description

technical field [0001] The present invention relates to a kind of in non-clinical research (safety evaluation) of medicine, utilizes the nuclear size morphology measurement method in dog testicular pachytene stage, leptotene stage and sperm cell identification and the evaluation of reproductive toxicity, this technology is based on measuring testicular The area and perimeter of the spermatogenic cell nucleus determine that the spermatocytes are in different stages of the cell cycle, and then judge the development of the spermatocytes according to the number and proportion of spermatocytes in each stage, and judge whether the drug is in the body of male dogs and other male animals. Effects on germ cells. It has the great advantages of being fast, accurate, and saving manpower and material resources. The invention provides an important evaluation basis for the toxicity evaluation of the reproductive system of dogs and other male animals in the non-clinical safety evaluation. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00
Inventor 徐勤邢成锋宋健平关业枝
Owner INST OF SCI & TECH GUANGDONG UNIV OF CHINESE MEDICINE
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