Agent for soil antibiotic resistance gene pollution reduction and its preparation and application

An antibiotic resistance and resistance gene technology, applied in the restoration, application, nitrogen fertilizer and other directions of polluted soil, can solve the problem of the decline in the production rate of resistance genes, and achieve the effect of maintaining the original function, optimizing the dosage, and reducing quickly

Active Publication Date: 2019-06-25
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, studies have also shown that quorum sensing is related to the formation of resistance genes in many microorganisms, and the rate of resistance gene production decreases significantly when quorum sensing is absent

Method used

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  • Agent for soil antibiotic resistance gene pollution reduction and its preparation and application
  • Agent for soil antibiotic resistance gene pollution reduction and its preparation and application
  • Agent for soil antibiotic resistance gene pollution reduction and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Preparation of natural plant extracts: mix lavender, fig leaves, dandelion, pineapple peel, dry rose, and pea shells in a mass ratio of 3:3:1:1:1:1, dry them, crush them with a grinder, and use 75 After extracting with % ethanol, filter through a 200-mesh sieve, collect the filtrate and plant residue, and obtain the filtrate by rotary evaporation.

[0056] (2) Preparation of quorum sensing inhibitor: 15 g of crude plant extract, 20 mg of pyrrolidone, and 25 mg of 2,5-diketopiperazine were uniformly mixed.

[0057] (3) Preparation of soil conditioner: the extracted plant residue is dried and mixed with carbonamide at a mass ratio of 100:1.

[0058] (4) Preparation of soil antibiotic resistance gene pollution reduction working agent: quorum sensing inhibitor and soil conditioner were evenly mixed at a ratio of 1:400 to obtain a resistance gene pollution reduction working agent.

[0059] (5) Apply soil antibiotic resistance gene pollution reducing agent: on the antibi...

Embodiment 2

[0062] (1) Preparation of natural plant extracts: mix lavender, fig leaves, dandelion, pineapple peel, dry rose, and pea shells in a mass ratio of 3:3:1:1:1:1, dry them, crush them with a grinder, and use 75 After extracting with % ethanol, filter through a 200-mesh sieve, collect the filtrate and plant residue, and obtain the filtrate by rotary evaporation.

[0063] (2) Preparation of quorum sensing inhibitor: 5 g of crude plant extract, 6 mg of pyrrolidone, and 5 mg of 2,5-diketopiperazine were uniformly mixed.

[0064] (3) Preparation of soil conditioner: the extracted plant residue is dried and mixed with carbonamide at a mass ratio of 100:1.

[0065] (4) Preparation of soil antibiotic resistance gene pollution reduction working agent: quorum sensing inhibitor and soil conditioner were evenly mixed at a ratio of 1:600 ​​to obtain a resistance gene pollution reduction working agent.

[0066] (5) Apply soil antibiotic resistance gene pollution reducing agent: on the antibio...

Embodiment 3

[0069] (1) Preparation of natural plant extracts: mix lavender, fig leaves, dandelion, pineapple peel, dry rose, and pea shells in a mass ratio of 3:3:1:1:1:1, dry them, crush them with a grinder, and use 75 After extracting with % ethanol, filter through a 200-mesh sieve, collect the filtrate and plant residue, and obtain the filtrate by rotary evaporation.

[0070] (2) Preparation of quorum sensing inhibitor: 25 g of crude plant extract, 35 mg of pyrrolidone, and 45 mg of 2,5-diketopiperazine were uniformly mixed.

[0071] (3) Preparation of soil conditioner: the extracted plant residue is dried and mixed with carbonamide at a mass ratio of 100:1.

[0072] (4) Preparation of soil antibiotic resistance gene pollution reduction working agent: quorum sensing inhibitor and soil conditioner were evenly mixed at a ratio of 1:100 to obtain a resistance gene pollution reduction working agent. process such as figure 1 shown.

[0073] (5) Apply soil antibiotic resistance gene pollu...

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Abstract

The invention discloses an agent for soil antibiotics resistance gene contamination reduction and preparation and application of the agent. A preparation method of the agent comprises the following steps that (1) at least one plant of dandelions, fig leaves, pineapple peels, roses, geraniums, lavender, pea hulls and rosemary is aired and then crushed, filtering is carried out after extraction, filter liquor and plant residues are collected, and the filter liquor is subjected to rotary evaporation to obtain natural plant crude extracts; and (2) 2-hydroxy-1,2-di-2-pyridylethanone, 2,5-diketopiperazine and the natural plant crude extracts are uniformly mixed according to the ratio to obtain a quorum sensing inhibitor, the plant residues and carbamide are mixed according to the ratio to obtain a soil improvement agent, the quorum sensing inhibitor and the soil improvement agent are uniformly mixed according to the ratio, and the agent can be obtained. The agent is directly applied to antibiotics resistance gene contaminated soil, or the agent is applied to the antibiotics resistance gene contaminated soil after being uniformly mixed with fertilizer, or the agent is applied to the antibiotics resistance gene contaminated soil before the fertilizer is applied.

Description

technical field [0001] The invention relates to the field of soil antibiotic resistance gene pollution, in particular to a method for reducing soil antibiotic resistance gene pollution. Background technique [0002] The status quo of soil antibiotic pollution: Antibiotic drugs have the effect of treating infectious diseases and increasing crop production, and are widely used in medical treatment, agricultural production and animal husbandry. The total annual consumption of antibiotics in China has reached a staggering 180,000 tons. These antibiotics cannot be fully absorbed and metabolized, and finally enter the soil environment through various channels, and their input has far exceeded their ecological self-purification level, which inevitably leads to the accumulation and enrichment of antibiotics in the soil. The detection rate of antibiotics in my country's soil is as high as 90% and above, and the average content of the most polluted tetracycline antibiotics is 200μg·k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/08C05G3/04C05G3/80
CPCB09C1/08B09C2101/00C05C11/00C05G3/80C05F11/00C05G3/00
Inventor 汪美贞严慧聪汪丽佳张芸芸申屠佳丽殷峻李娜沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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