Kit and method for testing glucose

A glucose and kit technology, applied in the field of medical testing, can solve problems such as inability to correctly evaluate glucose levels in diabetic patients, diabetic retinopathy, complications, etc.

Active Publication Date: 2017-01-11
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The interference of calcium dobesilate and phenetamine on enzymatic glucose detection is a major inconvenience to patients who are administered these two drugs
For example, for diabetic patients, diabetic retinopathy and varicose veins are often accompanied, and the interference of the administered calcium dobesilate on the enzymatic detection of glucose may cause the inability to correctly assess the glucose level of diabetic patients, thereby covering up and delaying the disease
For surgical patients, the interference of the administered enzymatic glucose detection by ethanol sulphate may make it impossible to correctly assess their glucose levels, resulting in postoperative complications and affecting the prognosis of patients
Therefore, this interference seriously affects the accuracy of the test results, affects the correct judgment of clinicians, and ultimately delays the treatment of patients, or causes casualties to patients by adopting unreasonable treatment measures
[0012] At present, the enzymatic detection kits sold on the market generally have the defect that they cannot effectively resist the interference of calcium dobesilate and phensulfonate

Method used

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  • Kit and method for testing glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1: the preparation of glucose assay kit

[0110] According to the components and concentrations shown in Table 14 and Table 15 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0111] Table 14: R1 formulation

[0112]

[0113] Table 15: R2 formulation

[0114]

[0115]

Embodiment 2

[0116] Embodiment 2: the preparation of glucose assay kit

[0117] According to the components and concentrations shown in Table 16 and Table 17 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0118] Table 16: R1 formulation

[0119]

[0120] Table 17: R2 formulation

[0121]

Embodiment 3

[0122] Embodiment 3: the preparation of glucose assay kit

[0123] According to the components and concentrations shown in Table 18 below and Table 19 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0124] Table 18: R1 formulation

[0125]

[0126] Table 19: R2 formulation

[0127]

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PUM

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Abstract

The invention relates to a kit and method for testing glucose. The kit comprises a reagent group 1 and a reagent group 2, wherein the reagent group 1 comprises ascorbic acid oxidase and chromogen; the reagent group 2 comprises 4-amino-antipyrine, peroxidase and glucose oxidase; the chromogen comprises N-ethyl-N(2- hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline or sodium salts of the N-ethyl-N(2- hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and the method provided by the invention can reduce the interference due to calcium dobesilate and / or etamsylate in a sample in the glucose determination.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting glucose, and more particularly relates to a glucose determination kit mainly used in automatic biochemical analyzers. Background technique [0002] Glucose (Glucose, Glu) is the most important carbohydrate present in human blood. Glucose (commonly known as blood sugar) in the blood is a necessary substance for human life activities, mainly derived from food digestion and absorption. [0003] Glucose (such as blood sugar) content must be maintained at a certain level to maintain the needs of various organs and tissues in the body, and its determination is of great significance for clinical testing. In general, the pathological increase of glucose is mainly seen in various diabetes, hyperthyroidism, adrenal hyperfunction, hyperfunction of basophils in the anterior pituitary gland, traumatic cranial trauma, intr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/54C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/54C12Q2326/96G01N2333/904G01N2333/908
Inventor 吴昌英罗岚
Owner SICHUAN MACCURA BIOTECH CO LTD
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