Detection method of ochratoxin a in capsicum extract

Abstract

The invention discloses a method for detecting ochratoxin A in capsicum extract. The method comprises the following steps: (1) performing ultrasonic extraction on a capsicum extract sample by using an alcoholic solution, so as to obtain an extracting solution; (2) freezing and centrifugally separating the extracting solution, so as to obtain the supernatant; (3) diluting the supernatant by using a PBS solution, and filtering to obtain the filtered liquid; (4) concentrating the filtered liquid to be dry, and adding a solvent for ultrasonic dissolving, so as to obtain a dissolving solution; (5) detecting the content of the ochratoxin A in the dissolving solution by adopting a liquid chromatography-fluorescence method. According to the method, toxins can be fully extracted from the sample, the impurities are removed by freezing and centrifuging, the sample is further purified by a PBS solution, an immunoaffinity column is not used, batch sample treatment is facilitated, and the effects of improving the detection efficiency, reducing the cost, saving energy and reducing consumption can be achieved. According to the method, the extracting solution is diluted and filtered by PBS and directly concentrated and detected, the detection limit is 1 [mu]g / kg, and the detection requirement is met.

Description

technical field

[0001] The invention belongs to the field of food safety detection, in particular to a method for detecting ochratoxin A in pepper extract. Background technique

[0002] Ochratoxins are a group of structurally similar toxic metabolites produced by toxin-producing strains such as Aspergillus and Penicillium, which are widely found in various foods, feeds and other agricultural by-products. Ochratoxins include 7 compounds with similar chemical structures, among which ochratoxin A (ochratoxin A, OTA) is the most widely distributed in nature, the most toxic, and has the greatest impact on humans, animals and plants. Toxicological studies have shown that ochratoxin A has renal toxicity, liver toxicity, immunotoxicity, teratogenicity, carcinogenicity and mutagenicity, etc., and has great potential harm to animal and human health. Therefore, all countries in the world attach importance to the detection and control of ochratoxin A, and have formulated relevant limit...

Images