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Recombinant turkey herpesvirus virus strain rHOH expressing H7N9 subtype avian influenza virus haemagglutinin protein and construction method

A technology for turkey herpes virus and avian influenza virus, which is applied in the field of recombinant virus vector vaccines, can solve the problems of decreasing maternal antibodies and reducing early protection of diseases, and achieves the effect of protecting health and safety

Inactive Publication Date: 2017-02-15
YANGZHOU UNIV
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Problems solved by technology

However, when using MDV to construct a recombinant virus, the heterologous strong promoter is less regulated by the virus. In the early stage of recombinant virus immunity, the protective antigen gene controlled by the promoter is expressed in large quantities, which is different from the maternal source of the antigen in the body. An antibody reaction that reduces maternal antibodies against that antigen, reducing the body's early protection against a disease

Method used

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  • Recombinant turkey herpesvirus virus strain rHOH expressing H7N9 subtype avian influenza virus haemagglutinin protein and construction method
  • Recombinant turkey herpesvirus virus strain rHOH expressing H7N9 subtype avian influenza virus haemagglutinin protein and construction method
  • Recombinant turkey herpesvirus virus strain rHOH expressing H7N9 subtype avian influenza virus haemagglutinin protein and construction method

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[0023] Material

[0024] rHVT-GFP and pCMGFP were constructed and preserved by the Key Laboratory of Livestock and Poultry Infectious Diseases of the Ministry of Agriculture of Yangzhou University, see patent application publication number: CN105002146A. H7N9 subtype avian influenza JX148 strain (A / chicken / Zhejiang / JX148 / 2014) was isolated and preserved by the Key Laboratory of Livestock and Poultry Infectious Diseases of the Ministry of Agriculture of Yangzhou University. The titer of chicken embryos was 10, and the hemagglutination inhibition reaction proved the polyantibody serum of JX148 It has high cross-reactivity with other subtypes of H7N9.

[0025] pCR2.1 vector: purchased from Invitrogen; High fidelity DNA polymerase, T4 DNA ligase, and Agarose Gel DNA Extraction Kit were purchased from Roche; the plasmid extraction kit (QIAprep SpinMiniPrep Kit) was a product of QIAGEN; other conventional reagents were domestically produced Analytical pure.

[0026] Step 1. Codon ...

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Abstract

The invention belongs to recombinant virus carrier vaccines in the field of molecular biology and biotechnology, and particularly relates to a recombinant turkey herpesvirus virus strain rHOH expressing H7N9 subtype avian influenza virus haemagglutinin protein and a construction method. The preservation number of the recombinant turkey herpesvirus virus strain rHOH is CGMCC NO.12985. A gB promoter of the recombinant turkey herpesvirus virus strain rHOH comes from a turkey herpesvirus virus carrier FC126 itself; an expressed heterologous antigen (haemagglutinin protein) gene is optimized through codon bias of Gallus Gallus, the nucleotide sequence changes, and no amino acid sequence changes. The recombinant turkey herpesvirus virus strain rHOH can be used for preparing vaccines for preventing H7N9 subtype avian influenza viruses; 18-day-old chick embryos obtained after hatch or 1-day-old chicks are inoculated, an H7N9 epidemic situation of poultry is prevented and controlled, and public health and safety are protected.

Description

technical field [0001] The invention belongs to the recombinant virus vector vaccine in the field of molecular biology and biotechnology, in particular to a recombinant turkey herpes virus (HVT) capable of expressing the hemagglutinin HA protein of H7N9 subtype avian influenza virus (Avian Influenza virus, AIV) The recombinant virus can be used for immunizing poultry to prevent H7N9 subtype avian influenza virus. Background technique [0002] The H7N9 subtype avian influenza that infects humans first broke out in the Yangtze River Delta region of East my country in 2013. It is a new type of avian influenza virus that has been recombined from three sources and is non-pathogenic or low-pathogenic to poultry. So far, it has spread to all parts of the country except Tibet and the Inner Mongolia Autonomous Region. The Pearl River Delta is another new source of outbreaks after the Yangtze River Delta. According to WHO statistics, as of June 13, 2016, there were 781 cases of human...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/869A61K39/145A61P31/16C12R1/93
CPCC07K14/005A61K39/00C12N15/86C12N2710/16021C12N2710/16043C12N2760/16122C12N2760/16134
Inventor 刘秀梵何东昌郝小利顾敏胡娇王晓泉刘晓文胡顺林
Owner YANGZHOU UNIV
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