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Method for obtaining explants of Quercus variabilis Bl tissue culture

A technology for tissue culture and acquisition methods, which is applied in the fields of botanical equipment and methods, culture medium, horticultural methods, etc., and can solve the problems that the sprouts of large trees are difficult to meet the needs of actual production and seedling cultivation, are difficult to become common materials for tissue culture, and are inconvenient to pick. , to achieve the effects of good explant quality, convenient mother plant management and sprout collection, and improved survival rate.

Inactive Publication Date: 2017-02-22
LIUZHOU SANNONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the large trees are located in remote forest areas, which are inconvenient to pick, difficult to germinate, small in number and difficult to sterilize. Therefore, it is difficult for large tree sprouts to meet the actual production and seedling needs.
However, isolated embryos, sterile seedlings, cotyledons and needles are used as explant materials, and the speed and quantity of adventitious buds obtained are not as fast and as many as stem segments, so it is difficult to become common materials for tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Select the seeds of the excellent family of Quercus cork with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 100-140 ° C. After natural cooling to room temperature, the seeds are sown in the Sand bed disinfected with potassium permanganate solution.

[0026] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, sprayed with water and sprayed according to the situation, and apply a compound with a mass concentration of 0.5% every 7-10 days. Fertilizer solution, wherein the active ingredient of NPK in the compound fertilizer solution is more than or equal to 48%, and the sprouts will take root after 40 days.

[0027] When the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 40-60 cm, a ground diameter of 0.3-0.6 cm, a straight trunk, and few side branches a...

Embodiment 2

[0030] Select the seeds of the excellent family of Quercus cork with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 110°C. Potassium acid solution disinfected sand bed.

[0031] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 8 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0032] After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 45-50 cm, a ground diameter of 0.3-0.4 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 cm × 30 cm and a substrate. Insi...

Embodiment 3

[0035] Select the seeds of the excellent family of Quercus cork with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 120°C. Potassium acid solution disinfected sand bed.

[0036] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15 cm × 15 cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 9 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0037]After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 50-55 cm, a ground diameter of 0.4-0.5 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 cm × 30 cm and a substrate. I...

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Abstract

The invention discloses a method for obtaining explants of Quercus variabilis Bl tissue culture. The method comprises the following processes: selecting seeds and sowing, transplanting and managing bud seedlings, planting and managing mother plants and obtaining the explants, planting the mother plants after being subjected to two-round selection of fine provenance and excellent strong seedlings, and cutting suitable sprouted branches from the mother plants to be sterilized and inoculated. According to the invention, the mother plants are managed and sprouted branches are collected both very conveniently, the obtained explants are excellent in quality, low in pollution rate and death rate, high in germination rate of adventitious buds, neat in sprouting and strong in operability, and meets the needs for establishing a rapid propagation system of the Quercus variabilis Bl tissue culture, so that the method for obtaining the explants of the Quercus variabilis Bl tissue culture has stronger economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant asexual propagation, and relates to a method for obtaining explants of cork oak tissue culture. Background technique [0002] cork oak ( Quercus variabilis Bl ) Also known as Cork Quercus, Quercus vulgaris, and Quercus vulgaris, it is a tree of the genus Quercus in the family Fagaceae, with a broad oval crown, many trunks, taupe, deep longitudinal fissures, and a thick cork layer. The branchlets are light brown, the male inflorescences are born in the lower part of the current year's branches, and the female flowers are solitary or twin in the axils of the current year's branches. The flowering period is May; the fruit matures in September-October of the following year. It is an important timber tree species in China. Cork oak likes light and is often born on sunny slopes in mountains, but it is better for young trees to have side shade. Strong adaptability to climate and soil. It can withstand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G9/10A01H4/00C05G3/00
CPCA01G17/005A01G24/00A01H4/00C05D1/00C05G5/40C05F11/00
Inventor 陈杏林
Owner LIUZHOU SANNONG TECH
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