High-density fermentation medium formula of sparassis crispa and pharmaceutical grade glucan preparation method of high-density fermentation medium formula

A technology for high-density fermentation and medium formulation, which is applied in the field of high-density fermentation medium formulation and pharmaceutical-grade glucan preparation of Emidiococcus, can solve the problem of Embroidery's wild field and limited yield, underdeveloped cultivation and breeding technology, artificial Harsh cultivation conditions and other problems, to achieve the effect of shortening the production cycle, easy operation, and clear ingredients

Inactive Publication Date: 2017-02-22
NINGBO XINUOYA MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wild land and yield of hydrangea are very limited, and the cultivation and breeding technology is not developed in China. The literature survey found that artificial solid cultivation is the most researched on the cultivation of hydrangea. The artificial cultivation conditions are harsh, the growth is slow, and the biotransformation rate is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The hydrangea strain (Sparassis crispa) of this example is preserved in the China Agricultural Microorganism Strain Resource Bank, and the strain code is ACCC 80458.

[0033] Carrying out high-density fermentation culture of Hydrangea ACCC 80458 and preparation of pharmaceutical grade dextran, the method includes the following steps:

[0034] Step 1: Preparation of Seed Liquid

[0035] Use the inoculation shovel to inoculate the preserved hydrangea on the PDA medium plate, and place it in a 28°C incubator to cultivate until colonies grow; use the inoculation shovel to dig out the full colony pieces from the above colonies and insert them into the SCF medium. 28°C, shake the flask at 150r / min for 12h to obtain the desired seed solution.

[0036] Step 2. Fermentation culture

[0037] Insert the seed solution prepared in step 1 into Hydrangea high-density fermentation medium with 10% inoculation amount to start fermentation culture, and the fermentation culture adopts 28...

Embodiment 2

[0044] The Hydrangea strain (Sparassis crispa) of this example is preserved in the China Agricultural Microorganism Strain Resource Bank, and the strain code is ACCC 51488.

[0045] Carrying out high-density fermentation culture and preparation of pharmaceutical grade dextran on hydrangea ACCC 51488, the method includes the following steps:

[0046] Step 1: Preparation of Seed Liquid

[0047] Use an inoculation shovel to inoculate the preserved hydrangea on a PDA medium plate, and place it in a 28°C incubator to cultivate until colonies grow; use the inoculation shovel to dig out full colony pieces from the above colonies and insert them into the SCF medium. 25°C, shake the flask at 100r / min for 12h to obtain the desired seed solution.

[0048] Step 2. Fermentation culture

[0049] Insert the seed solution prepared in step 1 into Hydrangea high-density fermentation medium with 10% inoculation amount to start fermentation culture, and the fermentation culture adopts 28°C, 150r / ...

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Abstract

The invention discloses a high-density fermentation medium formula of sparassis crispa and a pharmaceutical grade glucan preparation method of the high-density fermentation medium formula. The high-density fermentation medium formula comprises the following raw materials by weight percentage: 1-10% of soluble starch, 0.1-5% of peptone, 0.1-0.5% of KH2PO4, 0.1-0.5% of MgSO4.7H2O, and the balance of glucose solution at a mass concentration percentage of 20-50%, wherein the total weight percentage is 100%. A high biomass sparassis crispa mycelium is obtained by seed liquid preparation and fermentation culture. The pharmaceutical grade glucan preparation method comprises the steps of centrifuging and concentrating a sparassis crispa sporophore with a disc centrifuge, performing homogenization with a high-pressure homogenizer, and performing alkaline extraction, alcohol precipitation, sedimentation and drying to prepare pharmaceutical grade glucan. A glucan content in the mycelium detected by the method is 90%. Compared with the prior art, the formula and the method have the obvious advantages of short production cycle, low cost, simple technology, high product yield, high quality and the like.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a high-density fermentation medium formula of hydrangea and a preparation method of pharmaceutical-grade dextran. Background technique [0002] Hydrangea, belonging to the order of Non-Pleatophores, Hydrangeaceae, Hydrangea genus. The fruiting body is medium to large in shape, fleshy, with many branches from a thick stalk, and the ends of the branches form countless zigzag petals, which are named after the shape of a huge hydrangea. The biggest feature of hydrangea is that it contains a large amount of β-glucan. According to the analysis of the Japan Food Analysis Center, every 100g of hydrangea contains β-glucan as high as 43.6g, which is 3-4 times higher than that of Ganoderma lucidum and Agaricus blazei. It can be said that the β-glucan contained in hydrangea is the most among mushrooms, and it is known as the "king of mushrooms". β-glucan is a biologically ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/08C12R1/645
CPCC12N1/14C12P19/08
Inventor 田健施碧赢诸辉
Owner NINGBO XINUOYA MARINE BIOTECH CO LTD
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