Liver cancer detection primer probe and kit thereof

Abstract

The invention relates to a liver cancer detection primer probe and a kit thereof. The kit comprises a digital PCR premixed solution, a droplet stabilizer, a miRNAs primer probe mixed solution and a stem-loop reverse transcription primer mixed solution for RNA reverse transcription. The miRNAs primer probe mixed solution comprises an upstream primer and a probe which are used for detecting hsa-mir-21, hsa-mir-122, hsa-miR-199a and hsa-miR-1228, and a universal downstream primer used for detecting hsa-mir-21, hsa-mir-122, hsa-miR-199a and hsa-miR-1228. The stem-loop reverse transcription primer mixed solution comprises a stem-loop reverse transcription primer used for reverse transcription of hsa-mir-21, hsa-mir-122, hsa-miR-199a and hsa-miR-1228, wherein hsa-mir-21, hsa-mir-122 and hsa-miR-199a are markers, and hsa-miR-1228 is internal reference. The liver cancer detection kit can quickly, conveniently and accurately detect the expression level of miRNAs serving as liver cancer early diagnosis markers.

Description

technical field [0001] The invention relates to a primer probe set and a detection product for detecting the expression level of liver cancer miRNA, belonging to the field of biotechnology. Background technique [0002] microRNA is a kind of endogenous, highly conserved non-coding small molecule RNA, which is formed by the processing of single-stranded RNA precursor molecules, generally 18-23bp in length, and is first transcribed into the original miRNA under the action of RNA polymerase II during synthesis. Then it is cleaved by the Drosha enzyme of the ribonuclease III family to form the miRNA precursor, and then processed by the Dicer enzyme of the ribonuclease III family to finally form a mature miRNA. [0003] There are a large number of miRNAs in the human body. These mature miRNAs can bind to the RNA-induced silencing complex and bind to the 3' non-coding region of its target gene mRNA to regulate the translation process after transcription, thereby regulating the exp...

AI Technical Summary

Problems solved by technology

The results of sequencing technology are the most reliable, which is conducive to the discovery of new miRNAs, but the sequencing method is expensive

Although the price of miRNA chip is relatively low, its repeatability and accuracy are poor, so it is only used for primary screening of known disease-related miRNAs

The Northern Blot method is complicated and has low sensitivity, so it is not suitable for high-throughput detection of clinical samples

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