Kit for detecting testis carcinoma susceptibility and SNP (single nucleotide polymorphism) mark thereof

A marker and susceptibility technology, applied in the field of genetic testing, can solve the problems of lack of system integration, limitations, limited detection range, etc., and achieve the effect of good technical reproducibility, high practicability, and high cost performance.

Inactive Publication Date: 2017-03-08
深圳市核子基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0004] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample materials or lack of experience will lead to misdiagnosis of testicular cancer
Although other technologies such as imaging have been widely used in the examination and diagnosis of testicular cancer, they still have great limitations in the qualitative determination of the degree of testicular cancer

Method used

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  • Kit for detecting testis carcinoma susceptibility and SNP (single nucleotide polymorphism) mark thereof
  • Kit for detecting testis carcinoma susceptibility and SNP (single nucleotide polymorphism) mark thereof
  • Kit for detecting testis carcinoma susceptibility and SNP (single nucleotide polymorphism) mark thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 is used to detect SNP sites related to testicular cancer susceptibility

[0033] Among them, rs2900333 is located in the gene ATF7IP region, rs210138 is located in the gene BAK1 region, rs7040024 and rs755383 are located in the gene DMRT1 region, rs17021463 is located in the gene HPGDS region, rs995030, rs3782181 and rs4474514 are located in the gene KITLG region, rs12699477 is located in the gene NDF18IP region, and 20rs464 gene is located in the gene MAD18IP region. , rs7221274 is located in the gene PPM1E region, rs4888262 is located in the gene RFWD3 region, rs4635969 is located in the gene TERT region, rs9905704 is located in the gene TEX14 region, rs3790665 and rs4657482 are located in the gene UCK2 region.

Embodiment 2

[0034] Embodiment 2 is used for the test kit that detects testicular cancer susceptibility

[0035] 1. Preparation of the kit:

[0036] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 16 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0037] Table 1 PCR amplification primers and single base extension primers of SNP sites to be tested

[0038]

[0039]

[0040] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0041] 2. The detection method of the kit:

[0042] 1. Extraction of DNA

[0043] 1.1 DNA extraction from oral swab

[0044] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0045] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed...

Embodiment 3

[0092] The documents cited by 16 SNP sites closely related to testicular cancer in Example 3 are shown in Table 5

[0093] Table 5 Literature cited by 16 SNP sites closely related to testicular cancer

[0094]

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) mark for detecting testis carcinoma susceptibility. The SNP mark comprises 16 SNP sites including rs2900333, rs210138, rs7040024, rs755383, rs17021463, rs995030, rs3782181, rs4474514, rs12699477, rs4624820, rs7221274, rs4888262, rs4635969, rs9905704, rs3790665 and rs4657482. The invention also discloses a PCR (polymerase chain reaction) amplification primer of the SNP mark, a single-base extension primer and a kit thereof, and provides the important basis for the testis carcinoma disease catching risk evaluation and diagnosis reference.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting testicular cancer susceptibility and SNP markers thereof. Background technique [0002] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA sequence polymorphism caused by single nucleotide variation at the genome level. The frequency of occurrence in the population is greater than 1%. It is the most common type of human heritable variation. SNPs have strong genetic stability and are easy to detect. SNPs located inside genes can directly affect the structure or expression level of proteins, and then affect tissues, organs and even physiological functions. [0003] Early detection and systematic evaluation of multiple genetic markers associated with common dise...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor张核子
Owner深圳市核子基因科技有限公司