A microfluidic chip for single cell capture and culture

Abstract

The invention discloses a micro-fluidic chip for single cell capturing and culture. The micro-fluidic chip comprises an upper cover plate and a lower substrate, wherein micro-pore arrays are respectively arranged on opposite surfaces of the upper cover plate and the lower substrate; micro-pores of the upper cover plate and the lower substrate are aligned in a one-to-one manner; the micro-pores in the upper cover plate are distributed in an independent isolation manner; the micro-pores in the lower substrate are serially connected by a micro-flow channel in the lower substrate to form a liquid channel or are connected in parallel to form a plurality of liquid channels; a liquid inlet and a liquid outlet of each liquid channel are formed in the micro-fluidic chip; a micro baffle structure is arranged at the inlet of each micro-pore of the micro-pore array on the lower substrate; the micro baffle structure is used for capturing single target cells flowing into the micro baffle structure; a gap which is greater than the diameter of one target cell is formed between the micro baffle structure and the upper cover plate; and when the micro-fluidic chip is turned over, single target cells are captured by the micro baffle structure and are limited in corresponding micro-pores in the upper cover plate. The micro-fluidic chip is capable of achieving efficient capturing and culture of single cells, and is simple to manufacture.

Description

technical field [0001] The invention relates to a microfluidic chip for capturing and culturing single cells. Background technique [0002] Single-cell analysis has developed into a key issue in the study of cell function in the field of cell biology, and limited tools and technical support have made the isolation of single cells and subsequent single-cell research more and more prominent. The traditional way of extracting single cells is quite complicated: the cell suspension is dispensed into the culture plate, and the cells are allowed to settle, and then a microscope is used to confirm how many cells are successfully captured in the microwells on the plate. The field of view of the microscope cannot fit a single hole, so the researchers have to repeatedly move the plate, and repeat this process nearly a hundred times. This traditional serial dilution method is time consuming, laborious and inefficient. [0003] In recent years, the emergence of microfluidic chips has p...

AI Technical Summary

Problems solved by technology

However, its method of capturing cells through small micropores leads to low capture efficiency of single cells, and the probability of successfully obtaining a cell in a small hole is usually only 10% to 20%, and it needs to stand for a period of time during the operation, which makes it difficult to achieve single cell capture. Cell High Throughput Capture

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