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A Chromatographic Fingerprinting Method for Identification of Three Kinds of Medicinal Dendrobium

A technology of fingerprint and Dendrobium, applied in the field of chromatographic fingerprints for identifying three kinds of medicinal Dendrobium

Active Publication Date: 2018-09-14
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no public report on the use of HPSEC chromatography to establish the chromatographic fingerprint of medicinal Dendrobium polysaccharides

Method used

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  • A Chromatographic Fingerprinting Method for Identification of Three Kinds of Medicinal Dendrobium
  • A Chromatographic Fingerprinting Method for Identification of Three Kinds of Medicinal Dendrobium
  • A Chromatographic Fingerprinting Method for Identification of Three Kinds of Medicinal Dendrobium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A chromatographic fingerprint method for identifying three kinds of medicinal dendrobium, such as figure 1 shown, including the following steps:

[0041] (1): Polysaccharide extraction

[0042] The known three kinds of medicinal Dendrobium (Huoshan Dendrobium, Dendrobium officinale, Dendrobium slenderness) that are obtained by sampling in Dabie Mountains, Anhui Province according to the five-point sampling method are the fresh stems and defoliation treatment after wintering in the same year, Cut into thin slices and mix according to the type, then dried in an oven at 60°C to constant weight, pulverized into powder, extracted with anhydrous ethanol at 85°C Soxhlet until the reflux liquid is colorless, evaporated the solvent, and the obtained Dendrobium dregs According to the ratio of material to liquid 1:5, it was added into distilled water, and extracted by condensation and reflux in a water bath at 80°C for 4 times, each time for 4 hours; then, the above extraction fi...

Embodiment 2

[0065] A chromatographic fingerprint method for identifying three kinds of medicinal dendrobium, such as figure 1 shown, including the following steps:

[0066] (1): Polysaccharide extraction

[0067] The known three kinds of medicinal Dendrobium (Huoshan Dendrobium, Dendrobium officinale, Dendrobium slenderness) obtained by sampling in the Dabie Mountains of Anhui according to the five-point sampling method were mixed according to the type, and then dried to constant weight in an oven at 55°C, And pulverized into powder, Soxhlet extraction with anhydrous ethanol at 80 °C until the reflux liquid is colorless, evaporate the solvent, add the obtained Dendrobium dregs into distilled water according to the ratio of material to liquid 1:6, and extract 5 times by condensing and refluxing in a water bath at 80 °C , 3h each time; then, the above-mentioned extraction filtrate was combined and concentrated under reduced pressure to obtain a crude polysaccharide solution;

[0068] (2):...

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Abstract

The invention discloses a chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium. The method comprises the following steps of polysaccharide extraction, wherein a crude polysaccharide solution is acquired; polysaccharide purification and concentration, wherein a purified concentrated polysaccharide solution is acquired; chromatographic analysis, wherein a chromatographic fingerprint spectrum is established; unknown dendrobium variety identification. The method has the advantages that three varieties of dendrobium produced in Dabie Mountain in Anhui Province can be effectively distinguished and identified according to molecular weight distribution of the main medicinal component-dendrobium polysaccharides of the dendrobium, and defects existing in a dendrobium officinale identification method set in the 2015 edition of Chinese Pharmacopoeia and a dendrobium huoshanense identification method set in the 2016 edition of Anhui Provincial Standard can be overcome; due to the fact that the dendrobium officinale identification method and the dendrobium huoshanense identification method are rough overlaying information, counterfeiters can counterfeit the medicinal dendrobium through vulnerabilities of the detection methods, the method is closer to the essence of chemical components and is more detailed information, therefore, the counterfeiters hardly can counterfeit the medicinal dendrobium, or the imitation cost is high.

Description

technical field [0001] The invention relates to the technical field of identification and quality control methods for medicinal plant products of the genus Dendrobium orchid, in particular to a chromatographic fingerprint method for identifying three medicinal Dendrobium species. Background technique [0002] The Dabie Mountains of Anhui Province mainly produce 3 kinds of medicinal Dendrobium, the species names are: Dendrobium huoshanense C.Z.Tang et S.J.Cheng (also known as Dendrobium Huoshan), Dendrobium candidumWall.exLindl. ) and Dendrobium moniliforme (Linn.) Sw. (also known as Dendrobium copper-skinned). All three are Orchidaceae Dendrobium, which are perennial herbs. They mainly contain active ingredients such as polysaccharides, alkaloids, bibenzyl and phenanthrenes. The three were jointly declared by the Anhui Provincial Government as a geographical indication product "Huoshan Dendrobium" (the species name "Huoshan Dendrobium" refers to only one species, and the ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
CPCG01N30/06
Inventor 邓辉陈乃富陈存武陈乃东戴军
Owner WEST ANHUI UNIV
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