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Wild efficient expanding propagation method for arbuscular mycorrhizal fungi
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An arbuscular mycorrhizal fungus, field technology, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of heavy inoculant weight, inconvenient transportation, and less research
Inactive Publication Date: 2017-03-22
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Problems solved by technology
The soil matrix culture system is the most reliable and the most widely used inoculant production system, but it has the disadvantages of complex matrix components, sterilization treatment and heavy inoculant weight, which restricts the production of inoculants. Extension and application of arbuscular mycorrhiza
Therefore, it is of great significance to establish a high-efficiency propagation method for arbuscular mycorrhiza in the field, but there are few related studies at present.
Method used
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example 1
[0014] Example 1 Glomus mossea field propagation method
[0018] 1) First remove all soil surfaces and roots, stems, leaves, etc. of crops buried in the soil;
[0019] 2) Sprinkle the "big cleaning" granule on the surface of the soil to be disinfected;
[0020] 3) Deep plow the agent to the depth of sterilized soil of 20-30cm, break up large soil clods, and provide a good condition for the diffusion and penetration of decomposed gas in the "big sweep";
[0021] 4) Water until permeable, cover the soil surface with plastic film, and compact around the film to prevent air permeability;
[0022] 5) After 7-10 days of fumigation, uncover the film and ventilate for 10-15 days. During the ventilation period, loosen the soil 1-2 times a day;
[0031] 1) First remove all soil surfaces and roots, stems, leaves, etc. of crops buried in the soil;
[0032] 2) Sprinkle the "big cleaning" granule on the surface of the soil to be disinfected;
[0033] 3) Deep plow the agent to the depth of sterilized soil 20-30cm, break up large soil clods, and provide a good condition for the diffusion and penetration of decomposed gas in the "big sweep";
[0034] 4) Water until permeable, cover the soil surface with plastic film, and compact around the film to prevent air permeability;
[0035] 5) After 7-10 days of fumigation, uncover the film to ventilate for 10-15 days, and loosen the soil 1-2 times a day during the ventilation period;
[0036] 6) After ventilating for 10-15 days, sprinkle the arbuscular mycorrhizal fungal ag...
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Abstract
Arbuscular mycorrhiza is a mutual symbiont built by a kind of fungi in soil and a host plantroot system. Research shows that AM can participate in many physiological and biochemical metabolism processes of plants and have multi-aspect beneficial effects on the plants. Existing AM fungus expanding propagation production has the defects that a soil matrix culture system is mainly adopted, sterilization treatment and high inoculation agent weight are needed, and transportation is inconvenient, so that application and popularization of the arbuscular mycorrhizae are restricted. Therefore, construction of a wild efficient expanding propagation method for the arbuscular mycorrhizae has the important significance. The invention relates to a wild efficient expanding propagation method for arbuscular mycorrhizal fungi. The method comprises the steps that field soil is disinfected with dazomet, corn and sorghum are adopted as host plants, and a soil sample is taken to be detected after cultivation is conducted for 3-6 months under normal management, wherein the density of spores in the soil is higher than 200 / 10 g, and the hyphal length is larger than 150 m / 10 g.
Description
technical field [0001] The invention belongs to the technical field of biological bacterial agent production, in particular to a method for efficiently multiplying arbuscular mycorrhizal fungi in the wild. Background technique [0002] Arbuscular mycorrhiza (AM for short) is a mutualistic symbiosis established between a class of fungi in the soil and the roots of host plants, and belongs to endomycorrhizae. The vast majority of flowering plants in the world are mycorrhizal plants, of which arbuscular mycorrhizas (AM) account for about 90%. At present, AM is a hot topic in the field of microecology research. A large number of studies have shown that AM mycorrhizal can participate in many physiological and biochemical metabolic processes of plants, and has multiple effects on plants. In most cases, it is manifested as: rooting plants and improving the survival rate of transplanting; Promote the absorption of mineral nutrients by plant roots, especially the absorption of phosp...
Claims
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IPC IPC(8): A01G1/04
Inventor 黄璐琦陈美兰杨光陈敏李鹏英周修腾
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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