Breeding for brown planthopper population based on male sterilty after RNAi and control effect evaluation method

Abstract

The invention relates to breeding for brown planthopper population based on male sterilty after RNAi and a control effect evaluation method. According to the invention, a RNAi breeding method is employed for silencing brown planthopper male testis growth gene PHF7, by detecting the mortality and gene silencing rate, the concentration of dsNlPHF7 is determined; the silenced male insect growth and reproduction parameter indexes are detected, which mainly comprise body weight, life span, accessory gland protein content and arginine content; the copulated male insect growth and reproduction parameter indexes are detected, which mainly comprise body weight, the life span, vitellogenin content, oviposition amount, and YLS richness; and the expression level of the vitellogenin gene in the copulated male insect fat is detected. The brown planthopper population increasing and controlling effects enable quantification evaluation by detecting the important reproduction parameter indexes of the brown planthopper population male insects and female insects, the method has the advantages of simple and fast operation, and has wide applicability, the method provides a novel control approach for preventing and treating brown planthopper, and has theory meaning and utility values.

Description

technical field [0001] The invention relates to the indoor RNAi artificial breeding technology of brown planthopper, an important rice pest in my country, and a method for quantitatively evaluating its control effect. Background technique [0002] Brown planthopper Nilaparvata lugens It belongs to Hemiptera, planthopper family, is a seasonal, migratory, r-strategy, and explosive pest, widely distributed in Asia, distributed in South Asia, Southeast Asia, East Asia, South Pacific islands and Australia, starting from Pakistan in the west East to Japan and some islands in Southeast Asia, Micronesia and Papua New Guinea are also distributed. Brown planthopper is a monophagous pest, which can only feed and reproduce on rice and common wild rice. Since the 1960s, due to factors such as the promotion of dwarf rice varieties, the extensive use of N fertilizers and changes in other cultivation measures, and the intensive use of chemical pesticides, etc., frequent outbreaks of brow...

AI Technical Summary

Problems solved by technology

For a long time, multiple insecticides have been used alternately to control various rice pests, resulting in resistance in target pests and stimulation of reproduction in non-target pests

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