Reagent pre-packaged micro-fluidic chip, application method, experimental instrument and operating method
A microfluidic chip and pre-packaged technology, applied in the field of microfluidics, can solve the problems that cannot meet the requirements of chip stability and vibration resistance, and achieve the reduction of sample addition steps, rapid mass production, and low-cost Effect
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Embodiment 1
[0031] Such as figure 1 As shown, a reagent pre-packaged microfluidic chip of the present invention is arranged in multiple layers and includes from top to bottom: a reagent storage layer 1, an intermediate connection layer 2, a microchannel Layer 3, mixed reaction layer 4.
[0032] In the present invention, the reaction reagents are pre-packaged in the microfluidic chip, so that the conventional reagent kit is integrated and concentrated on a chip, and the sample adding step is reduced.
[0033] A number of reservoirs 101 are distributed on the reagent reservoir layer 1, and each reservoir 101 passes through the corresponding through holes of the intermediate connection layer 2 and the corresponding microchannels 301 in the microchannel layer to react with the mixed reaction layer 4 respectively. The pool 401 is connected.
[0034] The entrance of the through hole or microchannel is sealed by paraffin wax 5 (the paraffin wax is used as a valve and melted by heating to conduct the t...
Embodiment 2
[0041] On the basis of Example 1, the present invention also provides a method for using a reagent pre-packaged microfluidic chip, which includes the following steps:
[0042] Step S1, heating the reagent pre-packaged microfluidic chip 10, so that the reagent storage layer 1 and the mixing reaction layer 4 in the reagent pre-packaged microfluidic chip are connected; and step S2, pre-encapsulating the reagent microfluidic chip The upper surface of 10 is squeezed so that the reagent in the reagent storage layer 1 flows into the reaction tank 401 in the mixed reaction layer 4; and / or the air in the reaction tank 401 is drawn through a gas pipe 402, so that a negative reaction is formed in the reaction tank. The negative pressure allows the reagent in the reagent storage layer to flow into the reaction cell.
[0043] Specifically, the reagent pre-encapsulated microfluidic chip adopts a multi-layer arrangement, and from top to bottom are the reagent storage layer 1, the intermediate con...
Embodiment 3
[0051] Such as image 3 with Figure 4 As shown, on the basis of Example 1 and Example 2, the present invention also provides a reagent pre-packaged microfluidic chip experimental instrument, including: reagent pre-packaged microfluidic chip 10 for fixing the reagent pre-packaged microfluidic chip The microfluidic chip operating platform 11, the power supply module 122 for heating the reagent prepackaged microfluidic chip, and the reagent prepackaged microfluidic chip operating platform 11 is also provided with a prepackaged microfluidic controller suitable for extruding reagents The extruding device 12 on the upper end of the chip; the power supply module 122 and the extruding device 12 are all controlled by the processor module to control the extruding device to extrude the reagent pre-package when the reagent pre-packaged microfluidic chip is heated to a certain temperature The upper end surface of the microfluidic chip.
[0052] Specifically, the squeezing device 12 includes...
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