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Reagent prepackaged microfluidic chip, method of use, experimental apparatus and working method

A microfluidic chip and pre-packaged technology, applied in the field of microfluidics, can solve the problems that cannot meet the requirements of chip stability and vibration resistance, and achieve the effect of reducing sample addition steps, realizing cost, and low cost

Inactive Publication Date: 2018-01-26
SHANGHAI XUHAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the opening of prepackaged reagents is mostly realized by opening microvalves, which cannot meet the actual requirements for chip stability and vibration resistance.

Method used

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  • Reagent prepackaged microfluidic chip, method of use, experimental apparatus and working method
  • Reagent prepackaged microfluidic chip, method of use, experimental apparatus and working method
  • Reagent prepackaged microfluidic chip, method of use, experimental apparatus and working method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 As shown, a reagent prepackaged microfluidic chip of the present invention adopts a multi-layer arrangement, and includes from top to bottom: a reagent liquid storage layer 1, an intermediate connection layer 2, a microchannel Layer 3, mixed reaction layer 4.

[0032] The invention prepackages the reaction reagents in the microfluidic chip, so that the previous kits can be integrated and concentrated on one chip, and the steps of adding samples are reduced.

[0033] A number of liquid storage pools 101 are distributed on the reagent liquid storage layer 1, and each liquid storage pool 101 passes through the corresponding through holes of the intermediate connection layer 2 and the corresponding microchannels 301 in the microchannel layer to react with the reaction mixture in the mixed reaction layer 4 respectively. Pool 401 is connected.

[0034] The entrances of the through holes or microchannels are sealed with paraffin 5 (the paraffin is used as a ...

Embodiment 2

[0041] On the basis of Example 1, the present invention also provides a method for using a reagent prepackaged microfluidic chip, comprising the following steps:

[0042] Step S1, heating the reagent prepackaged microfluidic chip 10, so that the reagent liquid storage layer 1 in the reagent prepackaged microfluidic chip is connected to the mixed reaction layer 4; and step S2, heating the reagent prepackaged microfluidic chip The upper surface of 10 is extruded so that the reagent in the reagent liquid storage layer 1 flows into the reaction pool 401 in the mixed reaction layer 4; The negative pressure makes the reagent in the reagent storage layer flow into the reaction pool.

[0043] Specifically, the reagent prepackaged microfluidic chip adopts a multi-layer arrangement, and from top to bottom is the reagent liquid storage layer 1, the intermediate connection layer 2, the microchannel layer 3, and the mixed reaction layer 4, and each layer is adopted It is composed of a thi...

Embodiment 3

[0051] Such as image 3 and Figure 4 As shown, on the basis of Embodiment 1 and Embodiment 2, the present invention also provides an experimental instrument for a reagent prepackaged microfluidic chip, including: a reagent prepackaged for fixing a reagent prepackaged microfluidic chip 10 The microfluidic chip operating platform 11, the power supply module 122 for heating the reagent prepackaged microfluidic chip, and the reagent prepackaged microfluidic chip operating platform 11 is also equipped with a prepackaged microfluidic chip suitable for extruding reagents. The extruding device 12 on the end surface of the chip; the power supply module 122 and the extruding device 12 are controlled by the processor module, so that when the reagent prepackaged microfluidic chip is heated to a certain temperature, the extruding device is controlled to extrude the reagent prepackaged The upper surface of the microfluidic chip.

[0052] Specifically, the extruding device 12 includes: a ...

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Abstract

The invention relates to a reagent pre-packaged micro-fluidic chip, an application method, an experimental instrument and an operating method. The reagent pre-packaged micro-fluidic chip is arranged in multi-layers and comprises a reagent liquid storage layer, an intermediate connection layer, a micro-channel layer and a mixed reaction layer from the top to the bottom. A reaction reagent is pre-packaged in a micro-fluidic chip such that a sample adding step is reduced for an operator.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a reagent prepackaged microfluidic chip, a method of use, an experimental instrument and a working method. Background technique [0002] The microfluidic chip for biochemical detection is a multifunctional system chip, often called Lab-on-a-chip (Lab-on-a-chip), which combines sample preparation, quantitative injection, liquid mixing, biochemical reaction, The basic operation units such as separation and detection are integrated or basically integrated on a chip of a few square centimeters, which is a technical platform used to replace various functions of conventional chemical or biological laboratories. The basic feature and biggest advantage of microfluidic chips are the flexible combination and large-scale integration of various unit technologies on an overall controllable micro platform. Reagent prepackaging is an essential function of microfluidic chips in the field ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00B01L3/00
Inventor 沙俊李烽
Owner SHANGHAI XUHAN TECH CO LTD