Corn ear weight main effect qtl, its acquisition method and application

A corn, single-plant technology, applied in biochemical equipment and methods, recombinant DNA technology, sequence analysis, etc., can solve problems such as difficult to locate QTL, large QTL location confidence interval, and low marker density.
CN106636083BActive Publication Date: 2020-01-21LIUPANSHUI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
LIUPANSHUI NORMAL UNIV
Publication Date
2020-01-21

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Abstract

The invention provides a maize single panicle weight main effect QTL, as well as an acquisition method and application thereof. The QTL comprises qEW-1, qEW-2, qEW-3, qEW-4 and qEW-5. The method comprises the following steps: configuring cross combination by taking a high-yield early-maturity maize inbred line SG5 as a female parent and a maize inbred line SG7 with relatively poor yield trait as a male parent, and constructing an F2 genetic group of the cross combination; performing GBS sequencing-basing typing on the F2 genetic group, and performing genotyping by combining differential SNP validated based on SG5 and SG7 parents; investigating the yield trait of single F2 panicle, performing QTL analysis on the F2 single panicle yield trait by utilizing a winQTLcart2.5 software composite interval mapping method, and analyzing the chromosome region of the main effect QTL and the genetic effect. According to the method, a theoretic base is provided to excavating and controlling the maize single panicle weight main effect QTL and linked marker, a molecular marker closely linked with the target QTL is acquired, and foundation is laid to maize single panicle yield trait QTL candidate gene prediction, cloning and molecular mark assisted breeding.
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Description

technical field

[0001] The invention belongs to the technical field of molecular markers, and in particular relates to a major effect QTL for ear weight per corn plant, its obtaining method and application. Background technique

[0002] Corn is an important food and feed crop, one of the three major crops in the world, and my country's current corn has risen to the largest food crop. High yield is the eternal theme and direction pursued by maize breeders, and it is also an important goal of maize breeding. Yield traits are quantitative traits controlled by polygenes, a complex and diverse economic traits, and the ultimate manifestation of a series of physiological and biochemical processes controlled by interrelated polygenes. However, it is difficult to elucidate the specific reasons affecting the yield formation only by phenotypic analysis. Therefore, it is of great significance to understand the relationship between yield and related traits at the molecular level for th...

Claims

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