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Corn ear weight main effect qtl, its acquisition method and application

A corn, single-plant technology, applied in biochemical equipment and methods, recombinant DNA technology, sequence analysis, etc., can solve problems such as difficult to locate QTL, large QTL location confidence interval, and low marker density.

Active Publication Date: 2020-01-21
LIUPANSHUI NORMAL UNIV
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Problems solved by technology

[0005] In view of the problems in the prior art that the marker density of the genetic map used for the location of the QTL of maize yield is low, the confidence interval of the location of the QTL is relatively large, and it is difficult to directly predict the candidate genes for the location of the QTL, the present invention adopts the GBS simplified genome sequencing technology to construct a high-density SNP in maize Genetic map, combined with the investigated phenotypic traits of grain weight per ear in maize for genome-wide scanning, to obtain SNP molecular markers that are closely linked to the target trait QTL

Method used

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  • Corn ear weight main effect qtl, its acquisition method and application
  • Corn ear weight main effect qtl, its acquisition method and application

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Embodiment 1

[0020] The present invention uses the local high-yielding early-maturing corn inbred line SG-3 as the female parent and the relatively poor yielding inbred line SG-5 as the male parent to form a hybrid combination to construct its F2 population; perform GBS sequencing typing on the F2 population, At the same time, combined with the SNP markers developed between the two parents for genotype analysis, a genetic linkage map was constructed. Then investigate the yield traits per panicle of F2, and use QTLCartographer v2.5 software compound interval mapping method to analyze the QTL of yield traits per plant, and analyze the chromosomal region and genetic effect of the main QTL.

[0021] The inventive method specifically comprises the following steps:

[0022] 1. Parental selection and population construction: Select the high-yielding, early-maturing and gray spot-resistant maize inbred line SG-3 as the female parent, and the relatively poor-yielding inbred line SG-5 as the male pa...

Embodiment 2

[0043] Six maize lines with known ear weight per plant were selected, among which, 3 lines were high-yielding maize lines, and 3 maize lines were low-yielding maize lines.

[0044] The DNA genomes of the above-mentioned 6 lines were extracted respectively, and double-digested with restriction endonucleases MseI and HaeIII, and the DNA samples (6-leaf stage seedlings) were subjected to GBS sequencing.

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Abstract

The invention provides a maize single panicle weight main effect QTL, as well as an acquisition method and application thereof. The QTL comprises qEW-1, qEW-2, qEW-3, qEW-4 and qEW-5. The method comprises the following steps: configuring cross combination by taking a high-yield early-maturity maize inbred line SG5 as a female parent and a maize inbred line SG7 with relatively poor yield trait as a male parent, and constructing an F2 genetic group of the cross combination; performing GBS sequencing-basing typing on the F2 genetic group, and performing genotyping by combining differential SNP validated based on SG5 and SG7 parents; investigating the yield trait of single F2 panicle, performing QTL analysis on the F2 single panicle yield trait by utilizing a winQTLcart2.5 software composite interval mapping method, and analyzing the chromosome region of the main effect QTL and the genetic effect. According to the method, a theoretic base is provided to excavating and controlling the maize single panicle weight main effect QTL and linked marker, a molecular marker closely linked with the target QTL is acquired, and foundation is laid to maize single panicle yield trait QTL candidate gene prediction, cloning and molecular mark assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a major effect QTL for ear weight per corn plant, its obtaining method and application. Background technique [0002] Corn is an important food and feed crop, one of the three major crops in the world, and my country's current corn has risen to the largest food crop. High yield is the eternal theme and direction pursued by maize breeders, and it is also an important goal of maize breeding. Yield traits are quantitative traits controlled by polygenes, a complex and diverse economic traits, and the ultimate manifestation of a series of physiological and biochemical processes controlled by interrelated polygenes. However, it is difficult to elucidate the specific reasons affecting the yield formation only by phenotypic analysis. Therefore, it is of great significance to understand the relationship between yield and related traits at the molecular level for th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895G16B30/00
CPCC12Q1/6895C12Q2600/13C12Q2600/156G16B30/00
Inventor 苏成付
Owner LIUPANSHUI NORMAL UNIV
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