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Therapeutical agent for liver regeneration

A technology for liver regeneration and liver damage, applied in the direction of antineoplastic drugs, eicosan active ingredients, digestive system, etc., can solve the problems of no reports and applications

Inactive Publication Date: 2017-05-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Lubiprostone is a new anti-constipation drug, so far there is no report on its application in promoting liver regeneration

Method used

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  • Therapeutical agent for liver regeneration
  • Therapeutical agent for liver regeneration
  • Therapeutical agent for liver regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Select male C57BL / 6 mice more than eight weeks old, fix them on an anesthesia ventilator, anesthetize them with isoflurane, and perform 2 / 3 liver resection. The mice in the treatment group were orally dissolved with 5% sodium carboxymethylcellulose dissolved in lubiprostone (administration dose: 0.6 μg / kg). Respectively after 1, 2, 3, 4, and 7 days, the cervical spine was dismounted and killed, and the liver weight ratio of the control group and the oral lubiprostone group was observed, as shown in figure 1 As shown, it can be seen that the liver weight ratio of the oral lubiprostone group on the fourth day after the operation has a statistically significant increase compared with the control group (P<0.05 means significant). Biprostone can significantly promote liver regeneration after hepatectomy.

Embodiment 2

[0019] The mouse liver in Example 1 was soaked in formalin, the paraffin sections were fixed, and the paraffin sections were dewaxed, hydrated, antigen retrieved, blocked, incubated with the proliferation signal ki67 antibody, stained with DAB, dehydrated and mounted for microscopic observation, and counted for ki67 The number of positive signals, control group and oral lubiprostone were compared, and some slices were recovered three days after surgery as figure 2 shown. It can be seen that the ki67 in the oral lubiprostone group is significantly higher than that in the control group, indicating that oral administration of lubiprostone can enhance liver cell proliferation and promote liver regeneration.

Embodiment 3

[0021] The paraffin sections in Example 2 were stained with hematoxylin-eosin staining, and the deterioration of the fatty degeneration of the liver can be seen, such as image 3 shown. Among them, three animals were randomly selected each day for liver fatty change statistics as shown in the table below:

[0022] first day the next day third day fourth day seventh day control group 1 2 2 2 0 oral lubiprostone group 1 2 0 0 0

[0023] n=3

[0024] It can be seen from the above table that lubiprostone can make the fatty liver cells after hepatectomy in mice transform into normal liver cells faster, and can help the mice with liver injury after hepatectomy to restore the normal structure and function of the liver more quickly. Function.

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Abstract

The invention provides a pharmaceutical composition which utilizes lubiprostone as a main active ingredient and is capable of inducing hepatocyte proliferation and promoting liver regeneration after liver injury. The pharmaceutical composition can be a preparation based on oral administration, preferably, the pharmaceutical composition can be an oral solid preparation based on a tablet form or a capsule form. Furthermore, the pharmaceutical composition can be prepared into a lubiprostone unit preparation suitable for oral administration.

Description

technical field [0001] The invention relates to a pharmaceutical composition containing lubiprostone for inducing liver cell proliferation and promoting liver regeneration. Background technique [0002] The partially resected liver has the unique ability of short-term regenerative recovery (Nat Rev Mol Cell Biol2004, 5: 836; Adv Biochem Eng Biotechnol 2005, 93: 101), taking advantage of this property, liver resection has been widely used for some liver diseases application in clinical. Although hepatic resection has become safer over the past 10 years due to improvements in preoperative diagnosis, surgical technique, and postoperative care, in the presence of substantial liver disease, such as in patients with cirrhosis, severe hepatic steatosis, or colorectal liver metastases, debilitated by neoadjuvant chemotherapy prior to hepatectomy, impaired hepatocyte proliferation exposes patients to liver dysfunction and associated complications Liver failure is terminal, with hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/558A61P1/16A61P35/00
CPCA61K31/558
Inventor 孙宏斌赵文峰罗旱琼柳军
Owner CHINA PHARM UNIV
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