Application of compound microbial culture to third-time oil extraction

A technology of compound microorganisms and strains, which is applied in drilling compositions, chemical instruments and methods, etc., can solve the problems such as no research report on the application of oil-recovery microorganism strains, and can reduce the interfacial tension between oil and water and reduce the viscosity of crude oil. Effect

Inactive Publication Date: 2017-05-31
威海恒基伟业信息科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But for salinity greater than 10*10 4 mg/L (equivalent to NaCl content of 2.5mol/L) in high-salt res

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Application of composite microbial strains in the third oil recovery

[0014] Halobacterium salina ( Halobacterium salinarium ) Isolation of Hab-g03.

[0015] Halobacterium salina ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) The mixed fermentation broth of the three kinds of bacteria is applied in the third oil recovery according to the weight ratio of 1:1:1.

[0016] In the present invention, the Halobacter salinae ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) three kinds of bacteria can be purchased and obtained through public channels at home and abroad, and the obtaining of fermented liquid can also be seen through the prior art, but the prior art does not see the application of the three strains of fermented liquid in the third oil recovery.

Embodiment 2

[0017] Example 2: Application Effect Evaluation

[0018] The adopting halobacterium salina that provides for above-mentioned embodiment ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) according to the weight ratio of 1:1:1, the adaptability and oil recovery effect of the mixed fermentation broth in the high-salt oil recovery environment were evaluated, and the crude oil from the high-salt environment of Karamay oil production area and Qinghai Huatugou Jiandingshan and other places were evaluated. Oil displacement effect evaluation, evaluation indicators include: reduction of crude oil viscosity, reduction of interfacial tension between crude oil and formation water, and growth tolerance test, test method: add 60ml of crude oil, 90ml of formation water, and extreme salt-loving to a closed container Bacteria fermentation broth 1.5ml, add 3ml culture broth, at 35 ℃, shaker culture 8d. In the evaluation method, a...

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PUM

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Abstract

The invention discloses an application of a compound microbial culture to third-time oil extraction. A mixed fermenting liquid which is prepared halobacterium salinarium, bacillus brevis and bacillus cereus in a weight ratio of 1:1:1 is applied to third-time oil extraction. The culture survives in a high-salt environment, the salt content of which in a high salt oil extraction environment is higher than 12%. The viscosity of crude oil is reduced to separately reach 15.4%, 26.4% and 35.2%. The oil-water interfacial tensions are reduced to separately reach 91.3%, 76.5% and 58.6%. The compound microbial culture has beneficial effects of obviously reducing the viscosity of crude oil and the oil-water interfacial tensions and shows wide application value.

Description

technical field [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to the technical field of application of composite microbial strains in third oil recovery. Background technique [0002] In addition to the vast ocean (salt content 3.5-3.8%) on the earth, there are many salt ponds, salt lakes and saline-alkali lakes on the land. The salt content of the world-famous Dead Sea is as high as 31.5%. my country has a vast saline environment and rich resources of halophilic microorganisms. my country's Tibet, Qinghai, Xinjiang and Inner Mongolia have a large number of salt lakes and saline-alkali lakes with high salinity. The survey found that these salt areas have microbial growth, that is, the presence of halophilic bacteria. In the field of microbiology, halophilic bacteria usually refer to extreme halophilic bacteria, that is, microorganisms with an optimal salt concentration greater than 3.0m...

Claims

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Application Information

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IPC IPC(8): C09K8/582
CPCC09K8/582
Inventor 蔡颖
Owner 威海恒基伟业信息科技发展有限公司
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