Application of compound microbial culture to third-time oil extraction
A technology of compound microorganisms and strains, which is applied in drilling compositions, chemical instruments and methods, etc., can solve the problems such as no research report on the application of oil-recovery microorganism strains, and can reduce the interfacial tension between oil and water and reduce the viscosity of crude oil. Effect
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Embodiment 1
[0013] Example 1: Application of composite microbial strains in the third oil recovery
[0014] Halobacterium salina ( Halobacterium salinarium ) Isolation of Hab-g03.
[0015] Halobacterium salina ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) The mixed fermentation broth of the three kinds of bacteria is applied in the third oil recovery according to the weight ratio of 1:1:1.
[0016] In the present invention, the Halobacter salinae ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) three kinds of bacteria can be purchased and obtained through public channels at home and abroad, and the obtaining of fermented liquid can also be seen through the prior art, but the prior art does not see the application of the three strains of fermented liquid in the third oil recovery.
Embodiment 2
[0017] Example 2: Application Effect Evaluation
[0018] The adopting halobacterium salina that provides for above-mentioned embodiment ( Halobacterium salinarium ), Bacillus brevis ( Brevibacillus brevis ) and Bacillus cereus ( Bacillus cereus ) according to the weight ratio of 1:1:1, the adaptability and oil recovery effect of the mixed fermentation broth in the high-salt oil recovery environment were evaluated, and the crude oil from the high-salt environment of Karamay oil production area and Qinghai Huatugou Jiandingshan and other places were evaluated. Oil displacement effect evaluation, evaluation indicators include: reduction of crude oil viscosity, reduction of interfacial tension between crude oil and formation water, and growth tolerance test, test method: add 60ml of crude oil, 90ml of formation water, and extreme salt-loving to a closed container Bacteria fermentation broth 1.5ml, add 3ml culture broth, at 35 ℃, shaker culture 8d. In the evaluation method, a...
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