Kit and method for promoting in-vitro proliferation of radix campanumoeae

A technology of soil Codonopsis ginseng and box set, applied in the field of plant tissue culture, can solve the problems of low reproduction rate, yellowing of plants and unsustainable sub-generation reproduction.

Inactive Publication Date: 2017-06-09
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thus, the present invention adds thiadizuron (TDZ) for the first time and cooperates with 6-benzylaminopurine (BA) to induce the stem section with adventitious buds, and solves the problem of low reproduction rate and low The problem that plant yellowing cannot continue to be subcultured; the use of the box of the present invention can promote the repeated subculture of the stem section of Codonopsis pilosula for at least 5 times, and obtain a considerable number of aseptic Codonopsis pilosula seedlings after strong seedlings, rooting, and transplanting

Method used

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  • Kit and method for promoting in-vitro proliferation of radix campanumoeae
  • Kit and method for promoting in-vitro proliferation of radix campanumoeae
  • Kit and method for promoting in-vitro proliferation of radix campanumoeae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In October 2014, 5 fruits were collected from the greenhouse of South China Botanical Garden in Guangzhou. After in vitro germination, subculture, strong seedlings, rooting and transplanting, the specific steps are as follows:

[0050] (1) Fruit picking: On October 20, 2014, robust Codonopsis pilosula plants were selected in the greenhouse of the South China Botanical Garden in Guangzhou, and ripe but uncracked fruits were picked. They were taken back to the laboratory on the same day and stored in a 4°C refrigerator;

[0051] (2) Aseptic treatment of explants and induction of sterile seedlings: On October 21, 2014, on the ultra-clean workbench, the fruit was wiped with 75% alcohol for surface disinfection, and then the fruit was peeled off to take out the seeds. Direct inoculation on primary medium (30g / L sucrose and 7g / L agar added on the basis of MS medium) to induce seed germination. After 30 days, the germination rate of the seeds is close to 100%, and the plant he...

Embodiment 2

[0062] In October 2015, 5 fruits were collected from the greenhouse of South China Botanical Garden in Guangzhou. After in vitro germination, subculture, strong seedlings, rooting and transplanting, the specific steps are as follows:

[0063] (1) Fruit picking: On October 10, 2015, robust Codonopsis pilosula plants were selected in the greenhouse of South China Botanical Garden in Guangzhou, and ripe but uncracked fruits were picked. They were taken back to the laboratory on the same day and stored in a 4°C refrigerator.

[0064] (2) Aseptic treatment of explants and induction of sterile seedlings: On October 11, 2015, on the ultra-clean workbench, the fruit was wiped with 75% alcohol for surface disinfection, and then the fruit was peeled off to take out the seeds. Direct inoculation on primary medium (30g / L sucrose and 7g / L agar added on the basis of MS medium) to induce seed germination. After 30 days, the germination rate of the seeds is close to 100%, and the plant height...

example 3

[0073] Example 3 Effects of different concentrations of IBA and soaking time on the rooting of Codonopsis pilosula

[0074] Select the aseptic seedling of Codonopsis codonopsis cultivated in the medium 3 of Implementation Example 1, cut Codonopsis pilosula into 3-4 cm stem segments under aseptic conditions, and soak the far-growing end of the stem segment 0.2-0.5 cm away from the incision in In different concentrations of IBA, the soaking time was 10-15 minutes and then transferred to MS blank medium to observe the effects of different concentrations of IBA and different treatment times on the rooting of Codonopsis pilosula.

[0075] The test results are shown in the table below:

[0076]

[0077]

[0078]It can be seen from the above table that with the increase of the concentration of IBA, the number of lateral roots with a length greater than 1 cm of Codonopsis pilosula increased, and the concentration of IBA was positively correlated with the rooting rate. When the ...

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Abstract

The invention discloses a kit for promoting in-vitro proliferation of radix campanumoeae. The kit comprises a culture medium 1 used for inducing a stem section with adventives buds and a culture medium 2 used for repeated subculture, and each of the culture medium 1 and the culture medium 2 comprises 6-benzylaminopurine, thidiazuron and MS culture liquid. In addition, the invention discloses a method for promoting in-vitro proliferation of radix campanumoeae. By adopting the kit and the method, the problems that proliferated seedlings are thin, weak, withered and yellow and dead and proliferation rate is low when proportioning of NAA and BA is adopted for proliferation are solved; 30 days is taken as a culture cycle, proliferation rate of radix campanumoeae can reach 10-15 times by utilizing the kit, robust aseptic seedlings can be obtained, and either rooting rate or transplanting survival rate are 100%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a kit and a method for promoting the in vitro proliferation of Codonopsis pilosula. Background technique [0002] At present, there are few studies on rapid propagation of Codonopsis pilosula in vitro. Wang Zhunian et al. (2007) used mature fruits as explants, took seeds for aseptic germination to obtain seedlings, cut the seedlings into stems, and used MS+6-BA 1.0mg L -1 +NAA 0.1mg·L -1 , reproduce in a period of 20 days, the reproduction rate is 5-6 times, and then in the rooting medium MS+NAA 0.5mg·L -1 Rooting was carried out on the plant, and the rooting rate was 100%, and a complete plant was obtained after transplanting. We used the same method to aseptically germinate seeds to obtain seedlings, cut the seedlings into stems, and used MS+6-BA 1.0mg·L -1 +NAA 0.1mg·L -1 During propagation, the lateral buds of the inoculated stem segments germinated, but did...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/10A01G1/00
CPCA01G22/00A01G24/00A01H4/001A01H4/008
Inventor 胡秀冯敏许炳强梁韩枝其他发明人请求不公开姓名
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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