Automatic detection system for immunoblotting

Abstract

The invention discloses an automatic detection system for immunoblotting. The automatic detection system comprises an immunoblotting scroll, a liquid supply device and a waste liquid collecting device, wherein a plurality of stripy hydrophilic areas which are partitioned in parallel by hydrophobic areas are arranged on the outer surface in the circumferential direction of the immunoblotting scroll; the stripy hydrophilic areas are perpendicular to the central shaft of the immunoblotting scroll; the stripy hydrophilic areas have one or more conjunction areas in the length directions of the stripy hydrophilic areas; a plurality of liquid supply channels in one-to-one correspondence with the stripy hydrophilic areas are formed in the liquid supply device; water outlets of the liquid supply channels are formed relative to corresponding stripy hydrophilic areas; the stripy hydrophilic areas at the bottom of the immunoblotting scroll are connected with the water inlet of the waste liquid collecting device. By adopting the automatic detection system disclosed by the invention, the structure of the automatic detection system is simplified, in-situ detection can be achieved, and with the combination of adsorption of the stripy hydrophilic areas and target antibodies, reagents can be relatively well saved, and thus the automatic detection system can be applicable to trace detection.

Description

technical field [0001] The invention belongs to the field of protein analysis, and more specifically relates to an automatic detection system of immunoblotting. Background technique [0002] Western blotting (Western Blot) is a technique in which proteins are transferred to a membrane and then detected using antibodies. Western blotting is often used to identify a certain protein and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescence detection, the expression difference of the same protein in multiple samples can be compared at the same time. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay, Western blot has become a routine technique for protein analysis. [0003] In order to realize high-efficiency automated analysis, a variety of Western blot analyzers have emerged as needed (patent document CN103616526A). However, the above-mentioned western blot analyzer usua...

AI Technical Summary

Problems solved by technology

Therefore, it has the following disadvantages: 1. Due to the integration of a shaker for oscillating the reagent bottles, the structure of the entire analyzer is more complicated, thereby increasing the manufacturing cost; 2. The reaction conditions of different reagent bottles are slightly different, which affects the immunoblotting The accuracy; 3. The film needs to be taken out from the reagent bottle and dried before it can be detected, so that in-situ detection cannot be realized

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