Method for detecting estrogen pollution of water on basis of polymorphic vitellogenin of periophthalmus modestus

A mudskipper and estrogen technology, which is applied in the field of molecular biology and environmental pollution detection, can solve the problems of no patent report on environmental estrogen detection

Active Publication Date: 2017-06-13
SHANGHAI OCEAN UNIV +3
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some estrogen-like pollution detection methods have been developed for vitellogenin at home and abroad, but the selected fish m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting estrogen pollution of water on basis of polymorphic vitellogenin of periophthalmus modestus
  • Method for detecting estrogen pollution of water on basis of polymorphic vitellogenin of periophthalmus modestus
  • Method for detecting estrogen pollution of water on basis of polymorphic vitellogenin of periophthalmus modestus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] This embodiment relates to a mudskipper polytype vitellogenin (vtg) gene, an encoded protein and its application, and a method for cloning a full-length cDNA sequence.

[0035] VtgAa gene: The Mudskipper polytype VtgAa gene of the present invention is characterized in that the full length of the VtgAa sequence is 5223 bp, including the initiation codon ATG, the termination codon TAG and polyA. The open reading frame (ORF) of the gene is located at 3-5000 bp, with a length of 4998 bp, encoding a total of 1665 amino acids, of which the signal peptide is 15 amino acids. According to the calculation, the molecular weight of the encoded protein is 185kDa. The encoded amino acid sequence has a typical complete vitellogenin structure: a vitellogenin domain (located at amino acids 24-594 of the encoded amino acid peptide chain), a VWFD domain (located at amino acids 1423-1555 of the encoded amino acid peptide chain) ), a polyserine region (located at 1064-1195 amino acids of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for detecting estrogen pollution of water on the basis of polymorphic vitellogenin of periophthalmus modestus, and belongs to the field of molecular biology and environment pollution detection. Amino acid sequences of the vitellogenin of the periophthalmus modestus are shown as SEQ ID NO.2. The method has the advantages that the problems of deficiency of biological in-vivo detection for environmental estrogen by the aid of periophthalmus modestus and deficiency of corresponding biological detection technologies at present can be solved; vivo models of the Periophthalmus modestus which is high in tidal flat settlement property and sensitive to environments are selected, primers are designed, expression of the vitellogenin VtgAa of the periophthalmus modestus is detected by the aid of real-time quantitative fluorescent PCR (polymerase chain reaction) processes, and the method can be used for detecting estrogen pollution degrees of water environments such as seawater and mouths of rivers.

Description

technical field [0001] The invention belongs to the fields of molecular biology and environmental pollution detection, and in particular relates to a water environment estrogen detection method based on a novel tidal flat model fish-mudskipper polytype vitellogenin (vtg), which can be widely used in estuarine and tidal flat waters. Assessment of Environmental Estrogen Contamination Levels. Background technique [0002] In the contemporary era of rapid industrial development, the unreasonable release of a large number of chemical substances in the environment and the excessive dependence of modern agriculture on pesticides have caused great pollution to the environment. There is increasing evidence that there are a variety of substances in the environment that can mimic and interfere with the endocrine functions of animals and humans. After these exogenous compounds enter the body, they interfere with the synthesis, release, transport, metabolism, and combination of the body...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/46C12N15/12C12N15/10C12Q1/68
CPCC07K14/461C12N15/1096C12Q1/6851C12Q1/6888C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 吴美琴陈渊戈葛珂珂练青平
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap