Method for detecting estrogen pollution of water on basis of polymorphic vitellogenin of periophthalmus modestus

Abstract

The invention discloses a method for detecting estrogen pollution of water on the basis of polymorphic vitellogenin of periophthalmus modestus, and belongs to the field of molecular biology and environment pollution detection. Amino acid sequences of the vitellogenin of the periophthalmus modestus are shown as SEQ ID NO.2. The method has the advantages that the problems of deficiency of biological in-vivo detection for environmental estrogen by the aid of periophthalmus modestus and deficiency of corresponding biological detection technologies at present can be solved; vivo models of the Periophthalmus modestus which is high in tidal flat settlement property and sensitive to environments are selected, primers are designed, expression of the vitellogenin VtgAa of the periophthalmus modestus is detected by the aid of real-time quantitative fluorescent PCR (polymerase chain reaction) processes, and the method can be used for detecting estrogen pollution degrees of water environments such as seawater and mouths of rivers.

Description

technical field [0001] The invention belongs to the fields of molecular biology and environmental pollution detection, and in particular relates to a water environment estrogen detection method based on a novel tidal flat model fish-mudskipper polytype vitellogenin (vtg), which can be widely used in estuarine and tidal flat waters. Assessment of Environmental Estrogen Contamination Levels. Background technique [0002] In the contemporary era of rapid industrial development, the unreasonable release of a large number of chemical substances in the environment and the excessive dependence of modern agriculture on pesticides have caused great pollution to the environment. There is increasing evidence that there are a variety of substances in the environment that can mimic and interfere with the endocrine functions of animals and humans. After these exogenous compounds enter the body, they interfere with the synthesis, release, transport, metabolism, and combination of the body...

AI Technical Summary

Problems solved by technology

At present, some estrogen-like pollution detection methods have been developed for vitellogenin at home and abroad, but the selected fish models are mainly freshwater carps, and there are no patent reports on the use of fish in tidal waters for environmental estrogen detection.

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