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Culture medium for enterobacter sakazakii

A technology of Enterobacter sakazakii and culture medium, which is applied in the field of microbiology and can solve the problems of slow proliferation of Enterobacter sakazakii

Inactive Publication Date: 2017-06-13
ZHISHENG TIANJIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defects of the prior art, and provides a culture medium for Enterobacter sakazakii to solve the technical problem of slow proliferation of Enterobacter sakazakii in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A culture medium for Enterobacter sakazakii, consisting of the following components by weight: 8 parts of beef extract, 6 parts of oat flour, 2 parts of beet juice, 1 part of camellia, 1 part of kudzu flower, 1 part of bamboo leaf pepper, 1 part of cassia seed, 1 part of cowpea, 1 part of roasted licorice, and 1 part of mannitol. The medium is a liquid medium with a pH of 7.

Embodiment 2

[0017] A culture medium for Enterobacter sakazakii, consisting of the following components by weight: 8 parts of beef extract, 6 parts of oat flour, 2 parts of beet juice, 1 part of camellia, 1 part of kudzu flower, 1 part of bamboo leaf pepper, 1 part of cassia seed, 1 part of cowpea, 1 part of mannitol. The pH of the medium was 6.

Embodiment 3

[0019] A culture medium for Enterobacter sakazakii, consisting of the following components by weight: 8 parts of beef extract, 6 parts of oat flour, 2 parts of beet juice, 1 part of camellia, 1 part of kudzu flower, 1 part of bamboo leaf pepper, 1 part of cassia seed, 1 part of roasted licorice. The medium is a liquid medium with a pH of 8.

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PUM

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Abstract

The invention provides a culture medium for Enterobacter sakazakii, which comprises the following components by weight: 8 parts of beef extract, 6 parts of oat flour, 2 parts of beet juice, 1 part of camellia, 1 part of kudzu flower, 1 part of bamboo leaf pepper, 1 part of cassia seed; on this basis, a specific amount of cowpea, roasted licorice or mannitol can be added. On the basis of the commonly used materials in the culture medium, certain Chinese herbal ingredients are added to the formula, which unexpectedly achieves outstanding culture effects, especially suitable for the culture of Enterobacter sakazakii, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Enterobacter sakazakii. Background technique [0002] Enterobacter sakazakii (also known as Enterobacter sakazakii) is a type of Enterobacteriaceae, which was renamed Enterobacter sakazakii from Enterobacter cloacae in 1980. Enterobacter sakazakii can cause severe neonatal meningitis, enterocolitis and bacteremia, with a mortality rate as high as 50%. Currently, microbiologists do not know the source of E. sakazakii contamination, but many case reports indicate that infant formula is the main source of infection found so far. The strain has certain applications in the field of biotechnology, but the proliferation rate of Enterobacter sakazakii is slow in the commonly used culture medium, and at the same time, it is prone to bacterial contamination. Contents of the invention [0003] The present invention aims at the technical defects of the prior a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 季延滨李涛侯继明
Owner ZHISHENG TIANJIN BIOTECH
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