A method for accurate measurement of seed shattering property of Oryza sativa

A plant seed, precise measurement technology, applied in the direction of measuring device, strength characteristics, using stable tension/pressure test material strength, etc., can solve the problem of low accuracy, achieve accurate and reliable data, improve low accuracy, and result Objective and Accurate Effect

Active Publication Date: 2020-07-28
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The method for accurately measuring the shattering property of Oryza seeds provided by the present invention can improve the shortcomings of low accuracy of previous seed shattering detection methods, overcome errors that may be caused by human operations, and make the obtained seed shattering results more objective And accurate, so that the degree of seed shattering of different Oryza plants can be evaluated more accurately, which has important scientific research and production application value

Method used

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  • A method for accurate measurement of seed shattering property of Oryza sativa
  • A method for accurate measurement of seed shattering property of Oryza sativa

Examples

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Effect test

Embodiment 1

[0022] The method of the present invention is used to accurately identify the seed shattering properties of cultivated rice variety Minghui 86.

[0023] (1) Use the following steps to select a certain number of mature seeds of cultivated rice variety Minghui 86, use a digital display tension meter to measure the tensile force required for these seeds to fall, and calculate the average value of the tensile force ( BTS ave ):

[0024] At the maturity stage of the cultivated rice variety Minghui 86, 3 plants were randomly selected, and then 10 fully mature and plump seeds were randomly selected from each plant, and the digital display tension meter was used to measure the maximum resistance of these seeds during the shedding process. pull. The measurement method is: clamp the middle of the single seed on the ear of rice with a clamp closely connected to the digital display tension meter (using peak mode), and slowly pull the small cob in the horizontal direction until the seed falls...

Embodiment 2

[0036] The method of the present invention can accurately identify the seed shattering of weedy rice.

[0037] (1) Use the following steps to select a certain number of mature seeds of weed rice, use a digital display tension meter to measure the tensile force required for these seeds to fall, and calculate the average value of the tensile force ( BTS ave ):

[0038] Three plants were randomly selected during the maturity period of weed rice, and 10 fully mature and plump seeds were randomly selected from each plant. The digital display tension meter was used to measure the maximum tension that these seeds need to withstand during shedding. The measurement method is: clamp the middle of the single seed on the ear of rice with a clamp closely connected to the digital display tension meter (using peak mode), and slowly pull the small cob in the horizontal direction until the seed falls off. The value displayed by the tension meter at this time It is the pulling force required for a...

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Abstract

The invention belongs to the technical field of environmental biosafety detection and relates to a method for accurately measuring seed holding of rice plant seeds. The method comprises establishing a method for accurately measuring the average pulling force needed by the rice plant seed falling off and building a mathematical model for converting the measured pulling force into a seed holding index and concretely comprises accurately measuring pulling forces needed by the rice plant seed falling off through a digital display tension meter, calculating the average pulling force needed by falling-off of all detected seeds, and converting the average value into a seed holding index SHI according to a mathematical model, wherein seed holding is increased along with increasing of SHI and is decreased along with decreasing of SHI. The method can accurately and objectively measure seed holding degrees of different rice plants, provides a technical support for accurate screening of rice varieties with different seed holding degrees in scientific research and breeding and provides an effective detection method for seed mediated transgenosis diffusion analysis of rice wild sibling species subjected to transgenosis drifting.

Description

Technical field [0001] The invention belongs to the technical field of environmental biosafety detection, and specifically relates to a method for accurately measuring the seed shattering properties of Oryza plants. Background technique [0002] Seed shattering is a physiological phenomenon that plants naturally fall off after mature seeds, and it is an important trait essential for plants to reproduce themselves and spread offspring. Poaceae (Poaceae) rice plants ( Oryza ) In Asian cultivated rice ( O. sativaL .) is one of the most important food crops in the world, it was grown by its ancestors common wild rice ( O. rufipogonGriff .) Formed by artificial domestication and improvement. In the process of wild rice domestication, the seeds gradually lost their original natural shattering. However, its wild relatives (such as wild rice and weed rice) still maintain strong seed shattering. Some rice farm varieties have strong seed shattering ability. When rice is harvested, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N3/08
CPCG01N3/08G01N2203/0017
Inventor 卢宝荣晏焕新蔡星星
Owner FUDAN UNIV
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