Attention deficit hyperactivity disorder marker and application thereof and kit

A technology of ADHD and markers, applied in the field of medicine, can solve the problems of atomoxetine with few adverse reactions and slow onset time

Active Publication Date: 2017-06-27
WENZHOU KANGNING HOSPITAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Atomoxetine, listed as the first-line treatment for ADHD, has few adverse reactions and slow onset time, so it is not suitable for treating acute ADHD patients

Method used

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  • Attention deficit hyperactivity disorder marker and application thereof and kit
  • Attention deficit hyperactivity disorder marker and application thereof and kit
  • Attention deficit hyperactivity disorder marker and application thereof and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Identification of ghrelin mutants in zebrafish.

[0028] (1) Using TALEN to make mutants

[0029] A zebrafish ghrelin mutant is artificially produced, and its nucleotide sequence encoding ghrelin is shown in SEQ NO.2. Figure 1A It is the binding site of TALEN in this example.

[0030] The nucleotide sequence encoding ghrelin of wild zebrafish is SEQ NO.1, and the nucleotide sequence of the gene mutant is shown in SEQ NO.2. Compared with SEQ NO.1, the ghrelin gene mutant is missing 9 nucleotides (TGTGTCTCG), which was identified by sequencing to produce a stop codon, which can only encode MPLRCRASSMFLLLCVSLS*, but cannot synthesize the complete ghrelin precursor polypeptide . Individuals with mutants of this gene have decreased expression of the ghrelin precursor polypeptide. Studies have shown that individuals with this gene mutant will show symptoms of ADHD, so the expression of this gene mutant and the ghrelin precursor polypeptide can be used as a marker for ADHD...

Embodiment 2

[0041] Tracking of zebrafish behavior.

[0042] (1) In order to detect whether ghrelin mutants have ADHD-like phenotypes, 3-8 month-old adult zebrafish (control group) and ghrelin mutants were placed in separate fish tanks, (Ghrelin+ / - offspring produced by selfing The resulting ghrelin+ / + (AB or Control) and ghrelin- / - (ghrelin mutant) were self-crossed and the 3-8 month-old adult zebrafish were placed in a separate fish tank, and the viewpoint behavioral recorder was used to track the zebrafish Vitality, the total measurement time is 10 minutes, and the ordinate is the total exercise distance, Figure 2A .

[0043] Zebrafish vigor was tracked using a viewpoint behavioral recorder. The parameter setting on the behavior recorder is carried out according to the following table:

[0044] Adult fish behavior recording parameters

[0045]

[0046] Figure 2A is a comparison of adult male zebrafish ghrelin mutants and controls. Such as Figure 2A As shown, the movement di...

Embodiment 3

[0052]Behavioral tracking of zebrafish under photostimulation.

[0053] Adult zebrafish aged 3-8 months were placed in a separate fish tank, and the vigor of the zebrafish was tracked using a viewpoint behavioral recorder. The parameter settings on the behavior recorder are carried out as in Example 1, and the intensity and time of light stimulation are set in the following table:

[0054] Parameters for juvenile fish behavior recording

[0055]

[0056] Figure 3A Motility diagram of adult zebrafish under light stimulation. Figure 3B For the comparison of adult zebrafish activity under light stimulation. Such as Figure 3A and Figure 3B As shown, adult zebrafish locomotion increased under light stimulation. However, the movement distance of the ghrelin mutants was significantly increased compared with the control group under light stimulation.

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Abstract

The invention provides an attention deficit hyperactivity disorder marker and application thereof. The attention deficit hyperactivity disorder marker is ghrelin. The attention deficit hyperactivity disorder marker can screen potential patients suffering from attention deficit hyperactivity disorder through detection on peripheral blood / cerebrospinal fluid. A novel medicine for treating the attention deficit hyperactivity disorder, provided by the invention, provides novel therapeutic possibility and hope for the patients suffering from the attention deficit hyperactivity disorder.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to an ADHD marker and its application. Background technique [0002] ADHD, also known as attention deficit hyperactivity disorder (ADHD), is a common behavioral disorder in children. According to foreign reports, the prevalence of ADHD is between 5% and 10%, and the domestic survey is more than 10%. The intelligence of these children is normal or basically normal, but they have defects in learning, behavior and emotion. The main manifestations are inattention, short-term attention, excessive activities, emotional impulsiveness, and generally poor academic performance. After treatment, some children still have symptoms after they become adults, which obviously affects their studies, physical and mental health, family life and social skills in adulthood. [0003] Drugs can improve attention deficits, reduce activity levels, improve academic performance to some extent, and improve relationships...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/575G01N33/74A61K45/00A61P25/14
CPCA61K45/00C07K14/575G01N33/74G01N2333/575G01N2800/2835G01N2800/305A61K31/185A61P25/14
Inventor 李西殷战戴克智叶敏捷彭旭艳周贤用郑苗苗
Owner WENZHOU KANGNING HOSPITAL CO LTD
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