Analysis method for distinguishing true Rizhao green tea from fake Rizhao green tea on basis of UHPLC tandem high-resolution mass spectrum and by using metabonomic technology

Abstract

The invention discloses an analysis method for distinguishing true Rizhao green tea from fake Rizhao green tea on the basis of a UHPLC tandem high-resolution mass spectrum and by using the metabonomic technology. A Rizhao green tea sample and a to-be-detected green tea sample are subjected to pretreatment by means of organic solvents respectively, the ultra-high-efficiency liquid chromatography-quadrupole rod-rail trap high-resolution mass spectrum method is used for achieving separation and measurement of chemical components in the sample obtained after pretreatment, then UHPLC-MS original data of the Rizhao green tea sample and the to-be-detected green tea sample is preprocessed, and finally whether the to-be-detected green tea sample is the Rizhao green tea or not is distinguished by means of a main component analysis model by using the multivariate statistical analysis method. Analysis results are displayed in a PCA score plot mode. Technicians in the field can visually judge the true or false conditions of the Rizhao green tea, related verification and analysis do not need to be conducted to obtain true or false conclusions, and detection results are accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of tea industry quality detection, and in particular relates to an analysis method based on UHPLC tandem high-resolution mass spectrometry and applying metabolomics technology to distinguish the true holiday green tea. Background technique [0002] Metabolomics is the qualitative and quantitative analysis of all small molecule metabolites in a biological system at a given time and condition, so as to quantitatively describe the whole of biological endogenous metabolites and their response to internal and external changes. The object of metabolomics research is small molecule metabolites with a relative molecular mass of less than 1000, which can systematically and meticulously understand and understand the activity laws and metabolic processes of living organisms. [0003] The main research idea of ​​metabolomics is "global view", which focuses on the study of the overall situation of biological metabolites,...

AI Technical Summary

Problems solved by technology

There are hundreds of chemical components in tea such as water, alkaloids, enzymes, tea polyphenols, vitamins, and amino acids. These chemical components are also interrelated, and together they determine the quality of tea. The method of tea quality appraisal cannot qualitatively and quantitatively measure the chemical substances in tea. It is too subjective and gradually does not adapt to the current development of the tea industry economy. It is difficult to be convincing.

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