DKK-3 gene methylation diagnostic reagent system and kit, and applications thereof

Abstract

The invention belongs to the field of molecular diagnosis, and more specifically relates to a DKK-3 gene promoter region methylation diagnostic reagent system and a kit, and applications thereof. The DKK-3 gene promoter region methylation diagnostic reagent system comprises a probe sequence SEQ ID NO.1 used for detecting DKK-3 gene promoter region methylation, and a probe sequence SEQ ID NO.2 used for detecting DKK-3 gene promoter region non-methylation. The invention also provides the DKK-3 gene promoter region methylation diagnostic kit containing the DKK-3 gene promoter region methylation diagnostic reagent system, and applications of the DKK-3 gene promoter region methylation diagnostic reagent system or the DKK-3 gene promoter region methylation diagnostic kit in preparing reagents used for cervical carcinoma detection and / or diagnosis. Detection of DKK-3 gene promoter region methylation level is capable of guiding early stage diagnosis, treatment, and evaluating prognosis of cervical carcinoma effectively, and realizing in-time specific convenient diagnosis.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, relates to a reagent system for diagnosis, treatment and prognosis evaluation of cervical cancer, and in particular relates to a DKK-3 (Dipkkopf-related protein 3) gene methylation diagnosis reagent system, a kit and its application in Application in the field of reagents for cervical cancer detection and / or diagnosis. Background technique [0002] Cervical cancer is one of the most common female genital tract malignant tumors, and its incidence is second only to breast cancer, ranking second in female malignant tumors. According to worldwide statistics, there are about 500,000 new cases of cervical cancer every year, accounting for 5% of all new cancer cases, 80% of which occur in developing countries. About 240,000 people worldwide die of cervical cancer every year. There are about 130,000 new cases of cervical cancer and about 50,000 deaths in my country every year. Although the diagnosis ...

AI Technical Summary

Problems solved by technology

As people pay more and more attention to the influence of DNA methylation on the expression of genes related to tumorigenesis, the analysis of DNA methylation in various tumor diseases has become a research hotspot in the current molecular biology of tumors. However, the currently reported cancer There are many related genes, and the disease detection method based on DNA methylation often involves many genes, resulting in high cost and difficult to popularize

[0006] Dipkkopf-related protein 3 (DKK-3) is a newly discovered tumor suppressor gene and belongs to one of the DKK gene family members. The classic DKK family is considered to be a regulator of Wnt signaling pathway, but the function of DKK-3 is not yet complete. Clearly, most studies have shown that it has the effect of promoting cell apoptosis and tumor angiogenesis, and it shows a low expression state in a variety of tumor cells. When its methylation is silenced, it will lead to the loss of cell cycle regulation and canceration. Occurrence, progression and outcome are closely related

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