Method for acquisition of multi-bud paris polyphylla seedlings

A technology for seedlings and seedlings of Dianchia, applied in the field of seed biology, can solve the problems of inability to breed, long seedling breeding time, etc., and achieve the effect of shortening the emergence time

Inactive Publication Date: 2017-08-11
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0007] In order to solve the technical problem in the prior art that the single-bud Prunus diarrhoea seeds cannot be bred into multi-bud Prunus polyphylla seedlings, and the seedlings of single-bud Prunus polyphylla seeds have a long breeding time, the present invention provides different methods that can A method for obtaining multi-bud Chonglou seeds by treating single-bud Chonglou seeds into multi-bud Chonglou seedlings

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  • Method for acquisition of multi-bud paris polyphylla seedlings

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Embodiment 1

[0022] Embodiment 1 The inventive method

[0023] Main instrument of the present invention: artificial climate box (RGX-250).

[0024] A kind of method for obtaining polyphylla dianthus seedlings provided by the present invention, the steps are as follows:

[0025] (1) Put the mature single-bud Prunus diangi fruit to the outer testa to rot, rub off the outer testa of the single-bud Prunus diangi fruit to obtain the seeds, then dry the seeds in the shade indoors without direct sunlight, and dry them in the shade Put the seeds in a room without direct sunlight until there is no water residue on the surface of the seeds, and then perform sand storage and temperature-varying treatment. The sand storage and temperature-varying treatment is: bury the dried seeds in wet sand, and keep the humidity of the wet sand at 70%~ 75%, treated at 4°C for 30 days under dark conditions, and then treated at 20°C for 30 days (still under dark conditions);

[0026] (2) Sterilize the seeds treated...

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Abstract

The invention discloses a method for acquisition of multi-bud paris polyphylla seedlings, and relates to the technical field of the seed biology. The method comprises the following steps: collecting mature single-bud paris polyphylla fruits and placing until the scarfskin is corrupted, removing the testa, performing the variable temperature sand storage treatment to seeds of which the testa is removed, taking out after the seed germination, performing the asepsis under the aseptic condition, shifting to a medium of 1 / 2 MS + 0.5 mg / L of gibberellins, cultivating in 22 DEG C under the conditions of constant temperature and darkness, and cultivating under the conditions of 500 lx of light intensity, 12 h illumination / 12 h darkness, and 22 DEG C of the constant temperature, seeding in a seedbed after the germination, backfilling with soil, covering by pine needles, and constructing a sunshade net. The method is capable of combining the undiscovered method for changing the germination characters of the seeds with the traditional method for promoting the germination of the paris polyphylla seeds, and changing the single-bud paris polyphylla seeds to the multi-bud paris polyphylla seedlings through the different treatment conditions. The success rate is up to more than 95%.

Description

technical field [0001] The invention relates to the technical field of seed biology, in particular to a method for changing seeds from single-bud Prunus diatriana into multi-bud Prunus dioica seedlings by adopting different treatment conditions. Background technique [0002] Paris polyphylla var. yunnanensis (Paris polyphylla var. yunnanensis) is a perennial herb of the genus Polyphylla in the family Liliaceae, and is a medicinal plant species of Paris polyphylla included in the Pharmacopoeia. Its rhizome has the functions of clearing away heat and detoxification, relieving convulsions and relieving pain. It can be used for sore throat, convulsions in children, poisonous snake bites, boils and swollen toxins. The main raw material of Chinese patent medicines. For a long time, people have been relying on natural wild resources for the utilization of eucalyptus. Due to long-term excessive plundering and excavation, wild eucalyptus has been destroyed and is now on the verge of...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/02A01H3/04
CPCA01H3/02A01H3/04
Inventor 刘涛杨生超何霞红朱友勇李艳冰
Owner YUNNAN AGRICULTURAL UNIVERSITY
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