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Non-invasive body immunity function change monitoring method and application thereof

An immune, non-invasive technology, applied in the direction of chemical reaction of materials, material analysis and biological testing by observing the impact on chemical indicators, can solve the problem of detection materials and reagents, high cost, detection Long cycle and other problems, to achieve the effect of no security risk, low cost and convenient implementation

Inactive Publication Date: 2017-08-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common features of these immune function detection methods are invasive (blood collection), many test materials and reagents, long test cycle, high cost, and a normal control is required to judge whether the detected indicators are normal or abnormal.
More importantly, the strength of the body's immune function is the result of the combination of the detected indicators, and the above-mentioned single or several indicators cannot make a judgment on the strength of the immune function

Method used

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  • Non-invasive body immunity function change monitoring method and application thereof
  • Non-invasive body immunity function change monitoring method and application thereof
  • Non-invasive body immunity function change monitoring method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Immunocompromised mouse model and monitoring of glucose concentration in urine

[0031] Methods: Immediately after the beginning of the experiment, the model was established, and the immunocompromised mouse model was established. The mice in the model group (number of mice n=4) were intraperitoneally injected with cyclophosphamide 150 mg / kg to prepare the immunocompromised model. time, for four consecutive weeks; the normal group was modeled, and the mice in the normal group (number of mice n=7) were intraperitoneally injected with distilled water of the same volume; After 24 hours of urine, they were killed by dislocation of the cervical spine. The immune indicators were detected by conventional methods, and the relative concentration of glucose in urine samples was detected by NMR method.

[0032] Results: The modeling results of the immunocompromised mouse model and the normal group are shown in Table 1. figure 1 and Figure 2a ~ 2f shown. Among them, the comparis...

Embodiment 2

[0043] Immunoenhanced Mouse Model and Monitoring of Urine Glucose Concentration

[0044]Method: Modeling was started at the beginning of the experiment. The normal aged mice group (O group) and the normal aged mice gavage lentinan group (OA group), 7 mice in each group; 40mg / kg·day, gavage volume of 0.1mL / 10g, gavage once a day, continuous gavage for 30d; O group and OA group of mice normal intake and drinking water; after four weeks, the metabolism cage was collected 24h urine, the day before the end of the experiment, Then they were killed by dislocation of the cervical vertebrae, and the immune indexes were detected by conventional methods, and the relative concentration of glucose was detected by NMR method.

[0045] Results: The modeling test results of mice in group O and OA are shown in Table 3 to Table 5.

[0046] Table 3 Organ Index

[0047]

[0048]

[0049] Note: Compared with group O, △ P<0.05.

[0050] Table 4 Immune function index

[0051]

[0052] ...

Embodiment 3

[0063] Method: A subject collected 15ml of morning urine during the cold period, and collected 15ml of morning urine after the cold, which were marked as G1 and G2 respectively; 10ml of G1 and G2 urine samples were taken respectively, and centrifuged in a high-speed centrifuge at 5000g for 10min at 4°C , get supernatant 0.5ml, use the sulfuric acid phenol colorimetric method with pure glucose as reference substance, detect the glucose content in the supernatant.

[0064] Results: During the cold period, the glucose concentration in the urine sample G1 was 1.08 mg / ml, and the glucose concentration in the urine sample G2 after the cold was 0.78 mg / ml, and the glucose concentration decreased by 27.8%.

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Abstract

The invention discloses a non-invasive body immunity function change monitoring method and application thereof. The monitoring method includes steps: (1) collecting a urine sample; (2) detecting the content of glucose in the urine sample; (3) judging body immunity function changes, to be more specific, by taking the content of glucose in urine of a subject in a healthy state as a reference, increasing of the concentration of glucose in urine shows declining of body immunity functions; by taking the content of glucose in urine of the subject in a sub-healthy or diseased state, decreasing of the concentration of glucose in urine shows improving of the body immunity functions. The monitoring method which is non-invasive only requires collection of the urine sample, and low cost in sample acquisition, high compliance and freeness of safety risks are realized. Therefore, the method can be applied to early prediction of occurrence and development of immune hypofunction related diseases or sub-healthy state in medical treatment and health care or assessment of effects of immunity function intervention means.

Description

technical field [0001] The invention relates to the field of biomedical immunity monitoring, in particular to a non-invasive method for monitoring changes in body immune function and its application. Background technique [0002] In the same environment, some people catch a cold, while others act as if nothing had happened; some cancer patients are "declared" by doctors that they cannot live for a few months, but after many years, they are still alive. These phenomena are caused by differences in the function of the individual immune system. People usually refer to the body's resistance to foreign invasion, recognition and elimination of foreign matter as "immunity". [0003] The immune system takes on the tasks of defending against the invasion of foreign pathogens, monitoring mutations in the body, and removing aging and dead cells in the body. The body has a variety of immune responses, some are specific, some are non-specific; some are cellular immunity, some are humora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N21/78G01N30/02G01N24/08G01N21/00
CPCG01N33/48G01N21/00G01N21/78G01N24/08G01N30/02
Inventor 徐晓飞杨继国宁正祥
Owner SOUTH CHINA UNIV OF TECH
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