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A method for determining the content of multi-index components in Xingnaojing injection or its intermediate

A technology for Xingnaojing injection and a determination method, which is applied in the directions of measuring devices, material separation, and analysis of materials, can solve the problems of low detection cost, high detection sensitivity, and inability to accurately determine the content of various index components, and achieve detection cost The effect of low, good sensitivity, good application prospect and use value

Active Publication Date: 2019-06-11
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method has high detection sensitivity, simple operation, high practicability, and low detection cost. It can objectively, comprehensively and accurately evaluate the quality of Xingnaojing injection and its intermediates, and solve the problem of inability to accurately determine various index components due to the lack of reference substances. content problem

Method used

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  • A method for determining the content of multi-index components in Xingnaojing injection or its intermediate
  • A method for determining the content of multi-index components in Xingnaojing injection or its intermediate
  • A method for determining the content of multi-index components in Xingnaojing injection or its intermediate

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Experimental program
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Effect test

Embodiment 1

[0036] A method for assaying the content of multi-index components in an intermediate of Xingnaojing injection, the method comprising the following steps:

[0037] (1) Preparation of gardenia and turmeric steam distillation extract

[0038] The medicinal materials of gardenia and turmeric were crushed and sieved to 20-80 mesh respectively, 30g of powder was taken each, added with 1500mL of distilled water, and steam distillation was carried out until the volume of the distilled liquid was 1000mL, and the distillation was stopped to obtain the steam distillation and extraction of gardenia and turmeric liquid.

[0039] (2) preparation of need testing solution

[0040] Directly use the steam distillation extract of gardenia and turmeric obtained in step (1) (that is, the intermediate in the production process of Xingnaojing injection) as the test solution without pretreatment and dilution.

[0041] (3) Preparation of reference substance solution

[0042] Precisely weigh 21.67m...

Embodiment 2

[0077] A method for assaying multi-index component content in Xingnaojing injection, the method comprising the following steps:

[0078] (1) Preparation of test solution

[0079] Directly use Xingnaojing Injection as the test solution without pretreatment and dilution.

[0080] (2) Preparation of reference substance solution

[0081] Precisely weigh 16.25 mg of curcumadione reference substance, add mass fraction of 80% methanol aqueous solution to dissolve and dilute to 50mL, and make reference substance stock solution. Then accurately draw 1250 μL of the above-mentioned reference substance stock solution, add 30% methanol aqueous solution to dilute to 25 mL, and obtain a curcumedione reference substance solution with a concentration of 16.2 μg / mL, and store it at 4°C for future use.

[0082] (3) Determination of multi-index components in samples

[0083] Get the need testing solution that step (1) and step (2) obtain and curcumadione reference substance solution, sample in...

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Abstract

The invention discloses a measuring method of the contents of multiple index components in a Xingnaojing injection or an intermediate thereof. The method adopts a liquid chromatography analytic technique, and by the aid of a method of quantitative analysis of multi-components by a single marker, the contents of 9 index components in the Xingnaojing injection or the intermediate thereof can be simultaneously measured by using a curdione single reference substance. The method has high detection sensitivity and good accuracy, can evaluate the quality of the Xingnaojing injection or the intermediate thereof at lower cost, can solve the problem that the contents cannot be measured due to lack of the reference substances of the components to be measured, and is helpful to carry out quality control on the Xingnaojing injection more comprehensively.

Description

technical field [0001] The invention belongs to the field of content determination of traditional Chinese medicine components, and in particular relates to a method for determining the content of multi-index components in Xingnaojing injection or its intermediate. Background technique [0002] Xingnaojing Injection is an injection made of four traditional Chinese medicines: musk, turmeric, gardenia, and borneol. Impairment of consciousness, acute alcoholism and other diseases. The current quality standard of Xingnaojing Injection is the National Drug Standard in 2003 (WS 3 -B-3353-98-2003), this standard only identifies the muscone in the injection, carries out the content determination to the borneol, but does not carry out quality control for the composition in gardenia, turmeric. [0003] A number of documents have reported the content determination methods of muscone and borneol in Xingnaojing injection. There are literature reports on the content determination method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 严斌俊
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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