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Preparation method of oyster mushroom mother strain culture medium and oyster mushroom mother strain culture medium

A mother seed culture medium and technology of oyster mushrooms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of strain degradation, cellulose decomposition, hemicellulose ability decline, lack of cellulose, etc. , to achieve strong ability to decompose cellulose, improve the ability to decompose cellulose, and reduce the effect of metabolic activity

Active Publication Date: 2017-09-08
ZHUMADIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in conventional PDA culture medium, glucose is main carbon source, lacks the cellulose that is suitable for the growth of Pleurotus ostreatus mycelia in the culture medium, so the mother species of Pleurotus ostreatus is preserved in this medium for a long time, and Pleurotus ostreatus mycelia decomposes cellulose, The ability of hemicellulose is reduced, which eventually causes the degradation of the strain

Method used

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  • Preparation method of oyster mushroom mother strain culture medium and oyster mushroom mother strain culture medium

Examples

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Effect test

Embodiment 1

[0026] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0027] 1) A. Take 30g of fresh, mildew-free cottonseed hulls and 70g of corncobs, wrap them in four layers of gauze, soak in 500mL of water for 10 hours, then boil for 30 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0028] B. Take 160g of peeled and sliced ​​potatoes, put them in 500mL of water and boil for 30min, until the potatoes are cooked but not rotten, use four layers of gauze to filter to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 2

[0032] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0033] 1) A. Take 25g of fresh, mildew-free cottonseed hulls and 75g of corn cobs, wrap them in four layers of gauze, soak in 400mL of water for 6 hours, then boil for 20 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0034] B. Take 150g of peeled and sliced ​​potatoes, put them in 400mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 16 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 3

[0038] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0039] 1) A. Take 35g of fresh, mildew-free cottonseed hulls and 65g of corn cobs, wrap them in four layers of gauze, soak in 450mL of water for 8 hours, then boil for 40 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0040] B. Take 170g of peeled and sliced ​​potatoes, put them in 450mL of water and boil for 40min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 11g glucose and 17g agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45 minutes.

[0042] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

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Abstract

The invention relates to a preparation method of an oyster mushroom mother strain culture medium and the oyster mushroom mother strain culture medium and belongs to the technical field of culture medium preparation. According to the oyster mushroom mother strain culture medium prepared by the method, based on a common PDA culture medium, cooked filtered juice of cottonseed hulls and corncobs is added, a cellulose natural culture suitable for mycelial growth of oyster mushrooms is added, the content of potatoes, glucose and agar powder in raw materials are reduced, and metabolic action of mycelia of the oyster mushroom is favorably reduced, so that the variation speed of the oyster mushroom mother strain is reduced, and degeneration of excellent characteristics of the oyster mushrooms is prevented. By using the oyster mushroom mother strain grafted by the culture medium, the growth speed and growing vigor of the mycelia of the oyster mushrooms are obviously superior to those of the mycelia of the oyster mushrooms cultured by means of a conventional PDA culture medium.

Description

technical field [0001] The invention relates to a method for preparing a culture medium of oyster mushroom mother species and a culture medium of oyster mushroom mother species, belonging to the technical field of culture medium preparation. Background technique [0002] Edible fungus strains generally denominator species, original species and cultivated species. The parent species is a strain that is directly isolated from fruiting bodies (tissues or spores) and mushroom wood (ear wood). The medium is often nutrient-rich and easily absorbed potato agar medium (PDA medium). [0003] The mother species of Pleurotus ostreatus is generally preserved on an inclined plane. Generally, the mother species of Pleurotus ostreatus is stored in the refrigerator at 4°C. However, at this temperature, the metabolic activity of Pleurotus ostreatus hyphae has not stopped, and the metabolic activity of Pleurotus ostreatus mycelium is vigorous. The mutation rate is also faster. [0004] PDA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 魏银初班新河史红鸽李九英廖书荣潘正茂孙联合王红梅杨瑞晗
Owner ZHUMADIAN ACADEMY OF AGRI SCI
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