Preparation method of oyster mushroom mother strain culture medium and oyster mushroom mother strain culture medium
A mother seed culture medium and technology of oyster mushrooms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of strain degradation, cellulose decomposition, hemicellulose ability decline, lack of cellulose, etc. , to achieve strong ability to decompose cellulose, improve the ability to decompose cellulose, and reduce the effect of metabolic activity
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Embodiment 1
[0026] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:
[0027] 1) A. Take 30g of fresh, mildew-free cottonseed hulls and 70g of corncobs, wrap them in four layers of gauze, soak in 500mL of water for 10 hours, then boil for 30 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;
[0028] B. Take 160g of peeled and sliced potatoes, put them in 500mL of water and boil for 30min, until the potatoes are cooked but not rotten, use four layers of gauze to filter to obtain the filtrate, which is the potato juice;
[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.
[0030] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.
Embodiment 2
[0032] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:
[0033] 1) A. Take 25g of fresh, mildew-free cottonseed hulls and 75g of corn cobs, wrap them in four layers of gauze, soak in 400mL of water for 6 hours, then boil for 20 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;
[0034] B. Take 150g of peeled and sliced potatoes, put them in 400mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;
[0035] 2) Mix potato filtered juice and cellulose filtered juice with 16 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.
[0036] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.
Embodiment 3
[0038] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:
[0039] 1) A. Take 35g of fresh, mildew-free cottonseed hulls and 65g of corn cobs, wrap them in four layers of gauze, soak in 450mL of water for 8 hours, then boil for 40 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;
[0040] B. Take 170g of peeled and sliced potatoes, put them in 450mL of water and boil for 40min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;
[0041] 2) Mix potato filtered juice and cellulose filtered juice with 11g glucose and 17g agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45 minutes.
[0042] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.
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