A kind of preparation method of mushroom female seed culture medium and mushroom female seed culture medium
A technology of mother seed culture medium and shiitake mushrooms, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of strain degradation, functional degradation, variation, etc., and achieve the goal of decomposing cellulose Strong ability, easy operation, vigorous growth of mycelia
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Embodiment 1
[0026] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:
[0027] 1) A, take 95g of broad-leaved forest fine sawdust and 5g of bran, wrap them with four layers of gauze respectively, place them in 500mL of water and boil for 30min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;
[0028] B. Take 160g of peeled potatoes, put them in 500mL of water and boil for 30 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;
[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.
[0030] The medium of mushroom female species in this example was prepared by the above method.
Embodiment 2
[0032] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:
[0033] 1) A, take 90g of broad-leaved forest fine sawdust and 7g of bran, wrap them with four layers of gauze respectively, place them in 450mL water and boil for 20min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;
[0034] B. Take 150g of peeled potatoes, put them in 450mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;
[0035] 2) Mix potato filtered juice and cellulose filtered juice with 8 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.
[0036] The medium of mushroom female species in this example was prepared by the above method.
Embodiment 3
[0038] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:
[0039] 1) A, take 100g of broad-leaved forest fine sawdust and 3g of bran, wrap them with four layers of gauze respectively, place them in 450mL of water and boil for 25min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;
[0040] B. Take 170g of peeled potatoes, put them in 450mL of water and boil for 25min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;
[0041] 2) Mix potato filtered juice and cellulose filtered juice with 12g of glucose and 17g of agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45min.
[0042] The medium of mushroom female species in this example was prepared by the above method.
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