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A method for measuring chitosan concentration in aqueous solution

A technology for chitosan and water determination, which is applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of complicated operation steps and low analysis efficiency.

Active Publication Date: 2020-03-24
自然资源部天津海水淡化与综合利用研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] What the present invention aims to solve is the technical problem that the existing method for measuring chitosan content in water has complex operation steps and low analysis efficiency, and provides a method for measuring chitosan concentration in aqueous solution

Method used

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  • A method for measuring chitosan concentration in aqueous solution
  • A method for measuring chitosan concentration in aqueous solution
  • A method for measuring chitosan concentration in aqueous solution

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The chitosan sample of embodiment 1, embodiment 2, weight-average molecular weight is about 4 * 10 respectively 4 g / mol and 5×10 4 g / mol, analytically pure, Shanghai Macklin Biochemical Technology Co., Ltd.;

[0025] The chitosan sample of embodiment 3-6, weight-average molecular weight is about 7 * 10 respectively 4 g / mol, 1.3×10 5 g / mol, 2.0×10 5 g / mol, 3.5×10 5 g / mol, analytically pure, Beijing Bailingwei Technology Co., Ltd.;

[0026] Sodium hydroxide, glacial acetic acid, superior grade, Tianjin Kemiou Technology Co., Ltd.;

[0027] High-purity water, purified by MILLI-Q pure water system.

[0028] Example 1

[0029] Accurately weigh 5.0g with a weight average molecular weight of about 4×10 4 The chitosan sample of g / mol was dissolved with 100mL of 0.5% acetic acid aqueous solution, and after being completely dissolved to a clear solution, the volume was adjusted to 1.0L with pure water to prepare a chitosan with a concentration of 5.0g / L. stock solution. ...

Embodiment 2

[0032] Accurately weigh 5.0g with a weight average molecular weight of about 5×10 4 The chitosan sample of g / mol was dissolved with 100mL of 0.2% acetic acid aqueous solution, and after being completely dissolved to a clear solution, the volume was adjusted to 1.0L with pure water to prepare a chitosan with a concentration of 5.0g / L. stock solution. Add 0.25-10mL chitosan stock solution to a series of 50mL volumetric flasks respectively, and use 2.0g / L sodium hydroxide aqueous solution to dilute and shake up to obtain a series of chitosan standard series solutions with different concentrations. The range is 25-1000mg / L. Distilled water is the reference blank, and the turbidity of the above-mentioned chitosan standard series solutions is measured respectively with a turbidimeter, and the standard working curve is drawn with the concentration as the abscissa and the corresponding turbidity as the ordinate.

[0033] Get 5mL chitosan solution to be detected (wherein the weight a...

Embodiment 3

[0035] Accurately weigh 5.0g with a weight average molecular weight of about 7×10 4 The chitosan sample of g / mol was dissolved with 100mL of 1.0% acetic acid aqueous solution, and after being completely dissolved to a clear solution, the volume was adjusted to 1.0L with pure water to prepare a chitosan with a concentration of 5.0g / L. stock solution. Add 0.25-10mL chitosan stock solution to a series of 50mL volumetric flasks respectively, and use 5.0g / L sodium hydroxide aqueous solution to make up the volume and shake well to obtain a series of chitosan standard series solutions with different concentrations. The range is 25-1000mg / L. Distilled water is the reference blank, and the turbidity of the above-mentioned chitosan standard series solutions is measured respectively with a turbidimeter, and the standard working curve is drawn with the concentration as the abscissa and the corresponding turbidity as the ordinate.

[0036] The turbidity of the chitosan solution with the ...

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Abstract

The invention belongs to the technical field of water quality inspection and discloses a method for determining the concentration of chitosan in a water solution. The method comprises the following steps: dissolving a chitosan sample into an acetic acid solution, carrying out volume fixation by virtue of pure water so as to obtain a chitosan stock solution; carrying out volume fixation by virtue of a sodium hydroxide solution so as to obtain a chitosan standard series solution; respectively determining the turbidity of the chitosan standard series solution, and drawing a standard working curve by taking the concentration of the chitosan standard series solution as a horizontal ordinate and the corresponding turbidity as a longitudinal coordinate; and diluting a chitosan to-be-detected solution by virtue of the sodium hydroxide solution, determining the turbidity by virtue of a turbidity meter, solving the concentration of the diluted solution, and converting the concentration of the diluted solution into the concentration before the dilution. According to the method, the concentrations of chitosan water solutions with different molecular weights can be detected, and the concentration and turbidity of the solution present a good linear relation within a certain range; the relative standard deviation of the method is 0.75%-8.7%, the index recovery rate is 82.1%-122%, and the minimum detection limit is 2.1mg / L-4.8mg / L.

Description

technical field [0001] The invention belongs to the technical field of water quality detection, and in particular relates to a method for measuring chitosan concentration in an aqueous solution. Background technique [0002] Chitosan is the only alkaline polysaccharide in nature. It has biocompatibility, degradability and other functional activities. It has been paid more and more attention and favored by people. It has a wide range of uses in food, chemical industry, textile, medicine, environmental protection and other fields. Chitosan belongs to high polymer, and the weight-average molecular weight of naturally occurring chitosan in nature generally also varies from tens of thousands to millions, and the weight-average molecular weight of chitosan modified by chemical methods is generally in a wide range. The reported weight-average molecular weight range of chitosan is 5.0×10 2 -1.0×10 6 g / mol, and the molecular weight and molecular weight distribution are controllable...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/82
CPCG01N21/82
Inventor 刘静赵云荣曾兴宇周东星潘献辉
Owner 自然资源部天津海水淡化与综合利用研究所
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