Construction method of photoelectrochemical aptasensor based on gold/zinc oxide composite material and detection method for bisphenol A

An aptamer sensor and composite material technology, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of low sensitivity, general selectivity, low light utilization rate, etc., to achieve improved sensitivity and easy transition , high efficiency and high sensitivity

Inactive Publication Date: 2018-05-11
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for detecting bisphenol A include: gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and high-performance liquid chromatography, etc. However, these methods all have one or more of the following disadvantages: the method is complicated and the equipment is expensive , poor stability, low sensitivity, average selectivity, time-consuming, etc.
However, nano-zinc oxide has a relatively wide band gap, essentially low utilization rate of visible light, and high recombination rate of photogenerated electron-hole pairs to a large extent, which greatly limits its practical application in photocatalysis and photoelectron conversion in the future.

Method used

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  • Construction method of photoelectrochemical aptasensor based on gold/zinc oxide composite material and detection method for bisphenol A
  • Construction method of photoelectrochemical aptasensor based on gold/zinc oxide composite material and detection method for bisphenol A
  • Construction method of photoelectrochemical aptasensor based on gold/zinc oxide composite material and detection method for bisphenol A

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Construction of photoelectrochemical aptasensor based on gold / zinc oxide composite material,

[0030] First, the ITO conductive glass was cut into 1 cm×4 cm, cleaned and rinsed with a mixed solution of ammonia, hydrogen peroxide and deionized water at a volume ratio of 1:1:50, and then dried naturally at room temperature for use.

[0031] Then, 10 mL of Zn(NO 3 ) 2 ∙6H 2 O solution (0.5 M) was added dropwise to 10 mL of 4 M KOH solution under magnetic stirring, then the mixed solution was transferred to a 100 mL borosilicate glass bottle and reacted at 30°C for 12 h, the precipitate was collected by centrifugation, and the The mixture was alternately washed with absolute ethanol and pure water three times, and the washed precipitate was dried at 70°C to obtain pure zinc oxide substance.

[0032] Then, the appropriate amount of HAuCl 4 ∙4H 2 O (0.01 gmL -1 ) solution, in which the mass ratio of Au:ZnO was 7 wt% and 1mL methanol solution was added to 20 mL of pure...

Embodiment 2

[0038] The basic steps are the same as in Example 1, the difference is that an appropriate amount of HAuCl 4 ∙4H 2 O (0.01 gmL -1 ) solution, in which Au: ZnO was added to 20 mL of pure water according to a certain mass ratio and 1 mL of methanol solution, and 30 mg of the prepared zinc oxide was weighed and added to the mixture, stirred slowly for 1 h, and then the mixture was transferred to React in a 50 mL autoclave at 120°C for 1 h, cool naturally to room temperature, and finally collect the precipitate by centrifugation, wash the precipitate with absolute ethanol and deionized water alternately for 3 times, and the washed precipitate is at 70°C C conditions, and finally made gold / zinc oxide composite. The mass ratio of Au:ZnO in this step is 1wt%.

Embodiment 3

[0040] The basic steps are the same as in Example 1, the difference is that an appropriate amount of HAuCl 4 ∙4H 2 O (0.01 gmL -1 ) solution, in which Au: ZnO was added to 20 mL of pure water according to a certain mass ratio and 1 mL of methanol solution, and 30 mg of the prepared zinc oxide was weighed and added to the mixture, stirred slowly for 1 h, and then the mixture was transferred to React in a 50 mL autoclave at 120°C for 1 h, cool naturally to room temperature, and finally collect the precipitate by centrifugation, wash the precipitate with absolute ethanol and deionized water alternately for 3 times, and the washed precipitate is at 70°C C conditions, and finally made gold / zinc oxide composite. The mass ratio of Au:ZnO in this step is 3wt%.

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Abstract

The invention discloses a construction method of a photoelectrochemical adaptor sensor based on gold / zinc oxide composite and a method for detecting bisphenol A quickly and sensitively by using the photoelectrochemical adaptor sensor. Bar-shaped gold / zinc oxide composite is synthesized with a two-step method and is taken as a photoelectric beacon, the mass ratio of Au is adjusted to be 1%-10% by adjusting the proportion of dopant gold, the photoelectric beacon with excellent performance is prepared, the photoelectrochemical detection sensitivity is improved, and the concentration of bisphenol A can be effectively detected according to changes of photoelectric current. Equipment used for preparation of the photoelectrochemical adaptor sensor based on the gold / zinc oxide composite and used for detection of biphenol A is simple, the sample processing process is simple and convenient, and the construction method has the advantages of low cost, easiness in preparation and high efficiency and sensitivity.

Description

technical field [0001] The invention relates to a preparation method of a gold / zinc oxide composite material and a photoelectrochemical analysis and detection method for bisphenol A, belonging to the field of photoelectrochemical analysis. Background technique [0002] Bisphenol A (BPA) is a common chemical substance and is widely used as a main component of polycarbonate plastics in various polymer plastics such as: baby bottles, reusable water bottles, reusable plastic food containers, PVC film, paper and cardboard, etc. However, studies have shown that BPA is an endocrine disrupting chemical, and even exposure to low doses of BPA (approximately 10 -10 –10 -8 M) The cell proliferation of the human body and the health of the human body will also be significantly affected. At present, it is generally believed that the pollution of bisphenol A mainly comes from two aspects: (1) bisphenol A is released into the environment during the production of related products; (2) plas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/30G01N27/301
Inventor 乔云飞李红波王伟
Owner JIANGSU UNIV
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