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Quick detection method of Listeria monocytogenes in oyster

A monocytogenes, Listeria technology, applied in microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of false negative results, insufficient sensitivity, etc., and achieve high detection sensitivity , shorten the testing time, and ensure the effect of consumer health

Pending Publication Date: 2017-09-15
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a rapid detection method for Listeria monocytogenes in oysters, which solves the problem that conventional PCR and immunological detection methods in the prior art are not sensitive enough and prone to false negative results

Method used

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  • Quick detection method of Listeria monocytogenes in oyster
  • Quick detection method of Listeria monocytogenes in oyster
  • Quick detection method of Listeria monocytogenes in oyster

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Embodiment 1

[0036] A rapid detection method for Listeria monocytogenes in oysters, comprising the following steps:

[0037] S1, collect the sample to be tested

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Abstract

The invention belongs to the technical field of detection of food pathogenic bacteria and particularly discloses a quick detection method of Listeria monocytogenes in oyster; the method comprises the steps such as collecting a sample under test, treating the sample with cyclodextrin, preparing bentonite-coated activated carbon, preparing the sample treated via bentonite-coated activated carbon, and performing PCR (polymerase chain reaction) detection. The method of the invention has simple operating steps, is capable of effectively isolating pathogenic bacteria from foods, has no need for pre-enrichment, and takes 4 hours to detect a sample, the detection time is short, the detection limit is only 10 cfu / 25 g, the detection sensitivity is high, and false negative results rarely occur.

Description

technical field [0001] The invention belongs to the technical field of detection of food pathogenic bacteria, and in particular relates to a rapid detection method for Listeria monocytogenes in oysters. Background technique [0002] Foodborne pathogens are the primary cause of foodborne diseases. Foodborne pathogens cause great harm to human health and are a major hidden danger to food safety. Food-borne diseases do not decrease or disappear with economic development and technological progress. Vicious food safety incidents have occurred one after another worldwide. Food-borne diseases have not been effectively controlled. The incidence of food-borne diseases remains high and the food safety situation is severe. . Although the government supervision department continues to increase the supervision of food safety, the investment in food safety research has increased year by year, and food companies have also continuously improved food safety awareness in the production proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/6806C12Q1/686C12Q1/689C12Q2523/308
Inventor 杨洋张若鸿刘绍军蔡金星李军
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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