Solid lucid ganoderma strain and application thereof in submerged fermentation of lucid ganoderma

A liquid submerged fermentation and solid strain technology, applied in fermentation, fungi, and microbial-based methods, can solve the problems of slow growth of Ganoderma lucidum mycelium, long strain preparation cycle, and strain preparation failure, etc., to speed up fermentation Speed, increased fermentation growth points, and low requirements for production equipment

Inactive Publication Date: 2017-09-19
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ganoderma lucidum liquid submerged fermentation usually uses spheroid-type liquid strains as fermentation seeds, but the slow growth of ganoderma lucidum mycelium leads to a long period of strain preparation, and microbial contamination is prone to occur during operation and cultivation, resulting in failure of strain preparation , affecting production, and the fermentation process requires frequent preparation of liquid strains, the process is cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 adopts the liquid shaking flask fermentation process of Ganoderma lucidum solid bacterial classification

[0018] 1) Grind peanut shell powder and bran powder with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;

[0019] 2) Adjust the water content of the mixture to 60%, then take 100g and put it into a 250 mL Erlenmeyer flask, slightly compact the compost (leave a certain gap to facilitate ventilation), and seal it with a cotton plug;

[0020] 3) Sterilize the compost at 121°C for 2 hours;

[0021] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strain is obtained after the silk feed is complete;

[0022] 5) Under sterile conditions, mash the solid strain of Ganoderma lucidum with a sterile spoon, inoculate 0.2 g into a 250 mL shaker flask cont...

Embodiment 2

[0024] Embodiment 2 Adopt the submerged fermentation of the fermentation tank liquid of ganoderma lucidum solid strain

[0025] 1) Grind peanut shell powder and fine rice bran with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;

[0026] 2) Adjust the water content of the mixture to 60%, then take 300g and put it into a 1 L Erlenmeyer flask, slightly compact the compost (leave a certain amount of space to facilitate ventilation), and seal it with a cotton plug;

[0027] 3) Sterilize the compost at 121°C for 2 hours;

[0028] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strain is obtained after the silk feed is complete;

[0029] 5) Under sterile conditions, mash the solid strain of Ganoderma lucidum with a sterile spoon, take 10.5 g and inoculate it in a 10 L fe...

Embodiment 3

[0031] Example 3 Fermentation of Ganoderma lucidum liquid shake bottle using long-term preservation of solid strains

[0032] 1) Grind peanut shell powder and bran powder with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;

[0033] 2) Adjust the water content of the mixture to 60%, then take 100g and put it into a 250 mL Erlenmeyer flask, slightly compact the compost (leave a certain gap to facilitate ventilation), and seal it with a cotton plug;

[0034] 3) Sterilize the compost at 121°C for 2 hours;

[0035] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strains were prepared after the shredded food was complete, and stored in a 4°C freezer;

[0036] 5) Under aseptic conditions, take 0.2 g of Ganoderma lucidum solid strain preserved for 3 months with a sterile ...

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PUM

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Abstract

The invention discloses a solid lucid ganoderma strain and an application thereof in submerged fermentation of lucid ganoderma. Peanut shell powder and bran powder or fine rice bran are taken as raw materials and are prepared into compost, an activated lucid ganoderma slant strain is taken as a stock strain and the dark culture is performed in a strain bottle or a polypropylene plastic strain bag, so as to acquire the solid lucid ganoderma strain. Compared with the traditional liquid strain (primary in mycelium pellet), the solid lucid ganoderma strain disclosed by the invention has the advantages of low strain cost, long preservation time and direct application to liquid fermentation; the frequent preparation for the fermentation strain can be avoided and the increasing of the working efficiency is benefited; when the solid lucid ganoderma strain is used as the strain for the submerged fermentation culture of lucid ganoderma, the dispersity is high and a large amount of hypha growing points can be formed, so that the fermentation efficiency of the lucid ganoderma can be greatly promoted, the extracellular polysaccharide and biomass of the lucid ganoderma are obviously increased, the fermentation pollution rate can be reduced and the application prospect is extremely wide.

Description

technical field [0001] The invention belongs to the field of medicinal fungi and bioengineering applications, and in particular relates to a ganoderma solid strain and its application in ganoderma liquid submerged fermentation. Background technique [0002] Ganoderma lucidum ( Ganoderma lucidum ) is also known as Xiancao, Ruicao, Shenzhi, and not old grass. It belongs to Basidiomycetes, Agaricaceae, Polyporaceae, and Ganoderma fungus. It grows on the trunks and branches of different dead or growing broad-leaved trees. The ancients believed that It has the magical effect of bringing the dead back to life. Ganoderma lucidum in China mainly refers to lucidum with medicinal value, including red lucidum, purple lucidum, and pine and fir ganoderma. Ganoderma lucidum has a medicinal history of more than 2,000 years in my country and other oriental countries, and can be used for the treatment of various diseases such as hepatitis, hypertension, hyperlipidemia and gastric cancer. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12R1/645
CPCC12N1/14C12P1/02C12N1/145C12R2001/645
Inventor 刘盛荣张维瑞阮俊峰
Owner NINGDE NORMAL UNIV
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