Solid lucid ganoderma strain and application thereof in submerged fermentation of lucid ganoderma
A liquid submerged fermentation and solid strain technology, applied in fermentation, fungi, and microbial-based methods, can solve the problems of slow growth of Ganoderma lucidum mycelium, long strain preparation cycle, and strain preparation failure, etc., to speed up fermentation Speed, increased fermentation growth points, and low requirements for production equipment
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Embodiment 1
[0017] Embodiment 1 adopts the liquid shaking flask fermentation process of Ganoderma lucidum solid bacterial classification
[0018] 1) Grind peanut shell powder and bran powder with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;
[0019] 2) Adjust the water content of the mixture to 60%, then take 100g and put it into a 250 mL Erlenmeyer flask, slightly compact the compost (leave a certain gap to facilitate ventilation), and seal it with a cotton plug;
[0020] 3) Sterilize the compost at 121°C for 2 hours;
[0021] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strain is obtained after the silk feed is complete;
[0022] 5) Under sterile conditions, mash the solid strain of Ganoderma lucidum with a sterile spoon, inoculate 0.2 g into a 250 mL shaker flask cont...
Embodiment 2
[0024] Embodiment 2 Adopt the submerged fermentation of the fermentation tank liquid of ganoderma lucidum solid strain
[0025] 1) Grind peanut shell powder and fine rice bran with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;
[0026] 2) Adjust the water content of the mixture to 60%, then take 300g and put it into a 1 L Erlenmeyer flask, slightly compact the compost (leave a certain amount of space to facilitate ventilation), and seal it with a cotton plug;
[0027] 3) Sterilize the compost at 121°C for 2 hours;
[0028] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strain is obtained after the silk feed is complete;
[0029] 5) Under sterile conditions, mash the solid strain of Ganoderma lucidum with a sterile spoon, take 10.5 g and inoculate it in a 10 L fe...
Embodiment 3
[0031] Example 3 Fermentation of Ganoderma lucidum liquid shake bottle using long-term preservation of solid strains
[0032] 1) Grind peanut shell powder and bran powder with a traditional Chinese medicine grinder, and mix the two at a weight ratio of 8:2;
[0033] 2) Adjust the water content of the mixture to 60%, then take 100g and put it into a 250 mL Erlenmeyer flask, slightly compact the compost (leave a certain gap to facilitate ventilation), and seal it with a cotton plug;
[0034] 3) Sterilize the compost at 121°C for 2 hours;
[0035] 4) After the compost is cooled, insert a 2cm long and 1cm wide ganoderma slant strain on its surface under aseptic conditions, and then carry out dark culture at 25°C and relative humidity of 60-70%. The ganoderma lucidum solid strains were prepared after the shredded food was complete, and stored in a 4°C freezer;
[0036] 5) Under aseptic conditions, take 0.2 g of Ganoderma lucidum solid strain preserved for 3 months with a sterile ...
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