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Method for determining sex differentiation phase of Scophthalmus maximus by means of high temperature induction

A turbot and sex technology, applied in the field of fish farming, can solve the problems of high difficulty and high cost, and achieve the effect of low experimental cost

Inactive Publication Date: 2017-09-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0004] The invention provides a method for determining the sexual differentiation period of turbot by using high temperature induction to solve the problems of high cost and difficulty in existing determination methods

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  • Method for determining sex differentiation phase of Scophthalmus maximus by means of high temperature induction
  • Method for determining sex differentiation phase of Scophthalmus maximus by means of high temperature induction
  • Method for determining sex differentiation phase of Scophthalmus maximus by means of high temperature induction

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Embodiment

[0019] A method for determining the sexual differentiation period of turbot by high temperature induction, the steps of which are as follows:

[0020] (1) Constructing a full-sib family: select a female and a male common turbot with good maturity, obtain eggs and sperm respectively by artificial extrusion, place them in a dry and clean container, and use a dry method After artificial insemination, the fertilized eggs are rinsed and put into the hatching net cage for hatching under running water. The hatching water temperature is 13-15°C. After hatching, the larvae are transferred to the seedling cultivation pool for cultivation according to conventional methods, and the cultivation water temperature is 18±0.5°C.

[0021] (2) High temperature induction experiment: 18 glass fiber reinforced plastic water tanks with a volume of 200 L were set up, divided into 1 control group and 5 experimental groups, and each group had 3 repetitions. When the average full length of the 15-day-ol...

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Abstract

The invention provides a method for determining a sex differentiation phase of Scophthalmus maximus by means of high temperature induction. The method includes the steps of (1) building a full-sib family, (2) conducting a high temperature induction experiment and (3) determining the sex differentiation phase. According to the method, a control group and an experimental group where the other breeding conditions except water temperature and management are basically the same are set, larva fishes bred with the step (1) are stocked into water tanks of the experimental group and the control group respectively, the water temperature of the control group remains constant, a period of high temperature induction treatment is conducted on the experimental group at different developmental stages, and then cultivation is conducted on the experimental group and the control group where the water temperature remains the same until the sizes of fingerlings of the control group and the experimental group reach the sizes where sexes can be observed and identified through dissection; a relational graph of the male phenotype proportion of the experimental group and the fingerling size at the beginning of high temperature induction is drawn, wherein the fingerling size corresponding to the interval where a broken line of the male phenotype proportion changes obviously is the sex differentiation phase of Scophthalmus maximus. By means of the method, determination of the sex differentiation phase of Scophthalmus maximus is more accurate.

Description

technical field [0001] The invention belongs to the field of fish farming, and in particular relates to a method for determining the sex differentiation stage of turbot by using high temperature induction. Background technique [0002] The sex differentiation of fish refers to the process in which the primitive gonads with bidirectional development potential develop into testes or ovaries through a series of programmed events in the early developmental stages of dioecious fishes, some of which are accompanied by gonads Secondary sexual characteristics will also appear in the differentiation. Generally speaking, the sex determination method is mainly genetically determined dioecious fish. Although the basis of sex determination is genetic genes, it is easily affected by sensitive external factors such as temperature and exogenous hormones during the sex differentiation process, leading to the completion of final differentiation. The sex of a person is intersexual or a phenot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 刘新富孟振张和森刘滨胡鹏贾玉东杨志苏珂曲江波
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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