Method for identifying or screening disease-resistant rice material through rice blast seedling blast induced by field natural inoculation

Abstract

The invention provides a method for identifying or screening disease-resistant rice material through rice blast seedling blast induced by field natural inoculation, and belongs to the technical field of plant disease protection and rice breeding. The method provided by the invention comprises the following steps of (1) diseased straw collection and storage; (2) induction variety selection and use; (3) induction variety selection and seeding of a first batch induction variety; (4) seeding of materials to be identified and screened and a second batch induction variety; (5) diseased straw inoculation; (6) diseased seedling inoculation; (7) observation, recording and identification. Under the natural field conditions, the rice blast seedling blast is induced by inoculation of diseased straw and diseased seedlings. The method has the advantages that the rice seedling period growth is not influenced; the rice blast physiological strain basic number is great; the diversity is rich; the multi-time field large-scale application can be realized; the operation is simple and convenient; the cost is low; an identification result is accurate; the screening effect is obvious. The method can be popularized and applied to rice blast seedling blast resistance breeding identification and screening and rice blast study.

Description

technical field [0001] The invention relates to the technical field of rice resistance breeding and plant disease, in particular to a method for inducing rice blast seedling blast by natural inoculation in the field to identify or screen disease-resistant rice materials. Background technique [0002] Rice blast is one of the most serious diseases that harm rice, and the occurrence of rice blast in the seedling stage will greatly affect the growth of rice or even kill it, which will affect the production of rice in a large scale or even fail, seriously affecting the quality of rice high yield. [0003] Magnaporthe grisea is easy to mutate, and there are different physiological races. The diversity of physiological races or pathogenic types of Magnaporthe grisea is the result of host selection, and the power to produce races or pathogenic types mainly comes from spontaneous Mutation, large population of Magnaporthe grisea, rapid reproduction and high degree of variation are t...

AI Technical Summary

Problems solved by technology

[0005] In the experimental work related to the induction of rice blast, the current large-scale induction, identification, and screening of rice blast resistance breeding materials have problems such as untimely supply of the source of the blast fungus, insufficient supply, single pathogenic source of the blast fungus, Reasons such as weak pathogenicity, and artificial inoculation induce small scale and heavy workload, which makes the blast fungus strongly dependent on climate and season under the complex natural conditions in the field, which makes it difficult for the blast fungus to germinate and cause disease. Impact identification and screening effects

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