Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of hair follicle stem cells

A technology for hair follicle stem cells and plasmic stem cells is applied in the field of preparation of hair follicle stem cells, which can solve the problems of inability to meet ethical requirements and difficulty in obtaining raw materials.

Active Publication Date: 2020-05-26
GUANGZHOU RAINHOME PHARM&TECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a method for preparing hair follicle stem cells, which is used to solve the technical defects of difficulty in obtaining raw materials and inability to meet ethical requirements in the prior art.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of hair follicle stem cells
  • A kind of preparation method of hair follicle stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Medium A: Mix REGM and MEF medium at a volume ratio of 1:1.

[0036] Medium B: 450mLDMEM basal medium, 50mLFBS, 80pM insulin, 5mML-glutamine, 85ug / LbFGF, 15ug / LSCF and 3×10 -8 mol / L dexamethasone.

[0037] Medium C: 450 mL DMEM basal medium, 50 mL FBS, 10 ng / mL hEGF, 10 ug / LbFGF, 3 ng / mL HGF, 5 ng / mL PDGF and 5 ng / mL TGF-β.

[0038] Medium D: 450 mL DMEM basal medium, 50 mL FBS, 15 ng / mL hEGF, 10 ng / mL IGF, 0.5 ug / L hydrocortisone, 100 ng / mL linoleic acid, 4 ug / mL HGF, 150 ng / mL Wnt3a and 13 ug / mL KGF-7.

[0039] Medium E: 450 mL DMEM basal medium, 50 mL FBS, 0.015% TGF-β TGF-β, 10 ng / mL hEGF, 10 ng / ml VEGF, and 2.5 mmol / LL-glutamine.

[0040] Step 1. Collect urine cells

[0041] 1) Add 2mL penicillin / streptomycin double antibody to each collection cup.

[0042] 2) Collect the urine. If the follow-up operation is not performed immediately, store the urine in a 4°C refrigerator and complete the follow-up operation on the same day.

[0043] 3) One well of a six-well ...

Embodiment 2

[0065] Medium A: Mix REGM and MEF medium at a volume ratio of 1:1.

[0066] Medium B: 450mL DMEM basal medium, 50mLFBS, 1pM insulin, 1mML-glutamine, 50ug / LbFGF, 5ug / LSCF and 2×10 -8 mol / L dexamethasone.

[0067] Medium C: 450 mL DMEM basal medium, 50 mL FBS, 1 ng / mL hEGF, 100 ug / LbFGF, 1 ng / mL HGF, 10 ng / mL PDGF, and 10 ng / mL TGF-β.

[0068] Medium D: 450 mL DMEM basal medium, 50 mL FBS, 10 ng / mL hEGF, 1 ng / mL IGF, 1 ug / L hydrocortisone, 1 ng / mL linoleic acid, 5 ug / mL HGF, 30 ng / mL Wnt3a and 10 ug / mL KGF-7.

[0069] Medium E: 450 mL DMEM basal medium, 50 mL FBS, 0.001% TGF-β TGF-β, 1 ng / mL hEGF, 100 ng / ml VEGF, and 1 mmol / LL-glutamine.

[0070] Step 1. Collect urine cells

[0071] 1) Add 2mL penicillin / streptomycin double antibody to each collection cup.

[0072] 2) Collect the urine. If the follow-up operation is not performed immediately, store the urine in a 4°C refrigerator and complete the follow-up operation on the same day.

[0073] 3) One well of a six-well plate ...

Embodiment 3

[0095] Medium A: Mix REGM and MEF medium at a volume ratio of 1:1.

[0096] Medium B: 450mL DMEM basal medium, 50mL FBS, 100pM insulin, 10mML-glutamine, 200ug / LbFGF, 50ug / LSCF and 2.5×10-8mol / L dexamethasone.

[0097] Medium C: 450 mL DMEM basal medium, 50 mL FBS, 100 ng / mL hEGF, 1 ug / LbFGF, 50 ng / mL HGF, 20 ng / mL PDGF and 25 ng / mL TGF-β.

[0098] Medium D: 450 mL DMEM basal medium, 50 mL FBS, 115 ng / mL hEGF, 100 ng / mL IGF, 10 ug / L hydrocortisone, 150 ng / mL linoleic acid, 10 / mL HGF, 200 ng / mL Wnt3a and 65 ug / mL KGF-7.

[0099] Medium E: 450 mL DMEM basal medium, 50 mL FBS, 0.1% TGF-β TGF-β, 100 ng / mL hEGF, 1 ng / ml VEGF, and 10 mmol / LL-glutamine.

[0100] Step 1. Collect urine cells

[0101] 1) Add 2mL penicillin / streptomycin double antibody to each collection cup.

[0102] 2) Collect the urine. If the follow-up operation is not performed immediately, store the urine in a 4°C refrigerator and complete the follow-up operation on the same day.

[0103] 3) One well of a six-we...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biochemistry, and particularly relates to a preparation method of hair follicle stem cells. The invention provides the preparation method of the hair follicle stem cell. The method comprises the following steps of 1, collecting urine cells; 2, reprogramming urine cells; 3, inducing the induced culture of multifunctional cells; 4, performing induced culture of mesenchymal stem cells to obtain the hair follicle stem cells. Through the technical scheme provided by the invention, the hair follicle stem cells are prepared; the positive mark CK15 of the hair follicle stem cells is in positive expression in most cobblestone cells as hair follicle resources; meanwhile, through the analysis and display by a flow cytometry, the CK15, CK19, CD200 and beta 1 expression in the hair follicle stem cells is positive; the condition conforms to the expression result of the surface mark of the hair follicle stem cells. The preparation method of the hair follicle stem cells provided by the invention solves the problems of raw material obtaining difficulty and ethics requirement meeting incapability of the preparation method of the hair follicle stem cells in the prior art.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method of hair follicle stem cells. Background technique [0002] The skin is the organ with the largest surface area in the human body. It plays an important role in resisting microbial invasion, ultraviolet radiation, preventing water loss, and regulating body temperature. Limited skin volume and barriers to additional damage. [0003] The use of artificial skin cultured in vitro, that is, tissue engineered skin, as a source of transplantation can fundamentally solve this problem, but how to obtain seed cells has been puzzling people. With the development of medical biology and the deepening of skin tissue engineering, hair follicle stem cells have gradually attracted people's attention as a new seed cell. More and more studies have shown that hair follicle stem cells have the potential of multidirectional differentiation. However, in the prior ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10
CPCC12N5/0628C12N5/0666C12N2500/32C12N2501/11C12N2501/115C12N2501/125C12N2501/33C12N2501/39C12N2506/1376C12N2506/45C12N2510/00
Inventor 黄燕飞车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com