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Plant rooting and proliferation culture medium

A medium and basic medium technology, applied in the field of agriculture, can solve the problems of affecting the efficiency of test-tube seedling cultivation, weak growth of test-tube seedlings, underdeveloped root system, etc., and achieve the goals of shortening the induction rooting time, fast seedling growth, and strong plants Effect

Inactive Publication Date: 2017-10-17
山西景致苗木有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the test-tube plantlets obtained by tissue culture and gene transformation tend to grow weakly, have underdeveloped root systems, and have a low survival rate after transplanting, which seriously affects the cultivation efficiency and further application effect of test-tube plantlets.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A plant rooting and strong seedling medium, the formula of the medium is:

[0020] MS minimal medium + 0.04mg / L MnSO 4 Powder+0.75mg / L CuSO 4 Powder+0.06mg / L ZnSO 4 Powder + 0.34mg NiSO 4 Powder+0.35mg / L (NH 4 ) 6 Mo 7 o 24 .4H 2 O powder + 20mg / L EM original dew 100 times dilution + 2mg / L hymexazol + 4mg / L sugar alcohol chelated compound fertilizer + 5g / L agar powder + 20g / L sucrose + 3g / L Huabao No. 1+ 2g / L Huabao No.2.

Embodiment 2

[0022] A plant rooting and strong seedling medium, the formula of the medium is

[0023] MS basic medium + 0.06mg / L MnSO 4 Powder+0.95mg / L CuSO 4 Powder+0.08mg / L ZnSO 4 Powder+0.57mg NiSO 4 Powder+0.65mg / L (NH 4 ) 6 Mo 7 o 24 .4H 2 O powder + 30mg / L EM original dew 100 times dilution + 4mg / L hymexazol + 6mg / L sugar alcohol chelated compound fertilizer + 7g / L agar powder + 30g / L sucrose + 5g / L Huabao No. 1+ 4g / L Huabao No.2.

Embodiment 3

[0025] A plant rooting and strong seedling medium, the formula of the medium is

[0026] MS minimal medium + 0.05mg / L MnSO 4 Powder+0.85mg / L CuSO 4 Powder+0.07mg / L ZnSO 4 Powder + 0.455mg NiSO 4 Powder+0.5mg / L(NH 4 ) 6 Mo 7 o 24 .4H 2 O powder + 25mg / L EM original dew 100 times dilution + 3mg / L hymexazol + 5mg / L sugar alcohol chelated compound fertilizer + 6g / L agar powder + 25g / L sucrose + 4g / L Huabao No. 1+ 3g / L Huabao No.2.

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PUM

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Abstract

The invention discloses a culture medium for plant rooting and strong seedlings. The formula of the culture medium is: MS basic medium + 0.04-0.06mg / L MnSO4 powder + 0.75-0.95mg / L CuSO4 powder + 0.06-0.08mg / L ZnSO4 Powder+0.34‑0.57mg NiSO4 powder+0.35‑0.65mg / L(NH4)6Mo7O24.4H2O powder+20‑30mg / L 100 times dilution of EM original solution+2‑4mg / L hymexazol+4‑6mg / L Sugar alcohol chelated compound fertilizer+5‑7g / L agar powder+20‑30g / L sucrose+3‑5g / L Huabao No.1+2‑4g / L Huabao No.2. The invention effectively shortens the time for inducing rooting, the root system develops well, the plants are robust, and the transplanting survival rate is high.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a culture medium for plant rooting and strong seedling growth. Background technique [0002] Plant tissue culture technology is a technology developed in the early 20th century. It refers to the in vitro culture of isolated plant organs, tissues, cells, protoplasts, etc. under sterile conditions to induce callus, Adventitious buds, etc., followed by the process of forming a complete plant. Tissue culture technology, because of its advantages of controlling cell growth and differentiation under various artificial culture conditions, has effectively promoted the interdisciplinary development of plant physiology, pathology, pharmacy, genetics, breeding, and biochemistry, and has been widely used. In various industries such as agriculture, forestry, horticulture, industry, medicine, etc., it produces huge economic and social benefits. In summary, there are the following aspect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 谭志毅葛寒英雍鹏郝凯婕王平高行英
Owner 山西景致苗木有限公司
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