Peanut leaf tissue grinding method and peanut leaf tissue grinding device for extracting DNA

Abstract

The invention discloses a peanut leaf tissue grinding method and a peanut leaf tissue grinding device for extracting a DNA and belongs to the technical field of biotechnology. The tip of a pipette head is fired into a round ball through heating of an alcohol lamp; the pipette head of which the tip is fired into the round ball is matched with an existing centrifuge tube, and the peanut leaf tissues cooled through liquid nitrogen are mashed to achieve direct extraction of the DNA in the mashed peanut leaf tissues in the centrifuge tube. Compared with mashing of the peanut leaf tissues achieved by adopting a mortar and pestle coordination mode in the prior art, waste of the peanut leaf tissues in the grinding process is reduced and the peanut leaf tissue grinding method is more suitable for extraction of the DNA in the peanut leaf tissues when the peanut leaf tissues are relatively few; meanwhile, compared with an automatic sample grinding instrument in the prior art, the extraction cost of the DNA in peanut leaves is reduced and the usage amount of the peanut leaf tissues is reduced.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a peanut leaf tissue grinding method and device for extracting DNA. Background technique [0002] Peanuts, also known as groundnuts, are annual herbaceous plants, known as "vegetable meat" because of their high oil content. In addition to being used for food, peanuts can also be used in printing and dyeing, papermaking industries, etc., and are important oil crops and economic crops in my country. my country's peanut planting area and total production rank among the top in the world, and it has strong international competitiveness. However, my country's current peanut germplasm resources are relatively limited, and some germplasm resources that are resistant to disease and stress are relatively scarce, especially those resistant to Aspergillus flavus. [0003] Although conventional hybrid breeding and mutation breeding can improve the genetics of peanuts to a certain exte...

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Problems solved by technology

[0005] Because the mortar and pestle are used in the prior art to cooperate with each other to grind the peanut leaf tissue, a large amount of groundnut leaf tissue grinding fluid sticks to the mortar and pestle, resulting in waste of the peanut leaf tissue liquid. When it is less, the method in the prior art cannot be used to extract the DNA of peanut leaves

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