Method for producing double-staining plastic-sealed specimen of fish bones

Abstract

The invention discloses a method for producing a double-staining plastic-sealed specimen of fish bones. The method comprises the following conditions and steps: cleaning internal organs, scales and skin of fish bodies of fishes with hard and soft bones, fixing by virtue of 10% formalin, soaking with distilled water for 1-2 days so as to completely remove formalin, staining skeletons and soft bones blue by virtue of a dye, namely alcian blue, processing by virtue of alcohols of different concentrations, processing muscles of the fish bodies by virtue of trypsase until the muscles are in a transparent state, simultaneously maintaining the blue of the soft bones, staining the hard bones into red by virtue of alizarin red, carrying out re-transparentization by virtue of different concentrations of mixed solutions of 0.5%KOH and glycerin, plasticizing the fish bodies by virtue of polyvinylpyrrolidone, after the plasticization, carrying out air drying on the specimen, and finally sealing the specimen by virtue of epoxy resin. According to the finished specimen, the skeleton specimen including red hard bones and blue soft bones can be directly seen through the resin and the plasticized muscles.

Description

technical field [0001] The invention relates to a method for preparing a fish bone specimen, in particular to a method for preparing a fish bone double-dyed plastic-enclosed specimen. Background technique [0002] Fish skeleton specimens are part of ichthyology teaching and an important component of popular science education. Fish skeleton specimens can help students and children learn the knowledge of fish skeletal system. There are two traditional methods for making fish bones: one is to remove all the muscle parts of the fish and reassemble the bones to their original state. The production process is cumbersome, and it is easy to lose some small bones or the bones are assembled incorrectly. The production of bone specimens Less efficient, incomplete specimens of fish skeletons also reduce the educational value. The second is to store the specimen in glycerin after a series of transparent and staining processes, but liquid glycerin is not easy to store and transport. At...

AI Technical Summary

Problems solved by technology

There are two traditional methods for making fish bones: one is to remove all the muscle parts of the fish and reassemble the bones to their original state. The production process is cumbersome, and it is easy to lose some small bones or the bones are assembled incorrectly. The production of bone specimens Less efficient, incomplete specimens of fish skeletons also reduce educational value

The second is to preserve the specimen in glycerin after a series of transparent and staining processes, but liquid glycerin is not easy to preserve and transport