Construction method and applications of tussilago farfara l liquid phase fingerprint

A technology of fingerprint spectrum and construction method, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as reflecting the quality of medicinal materials, and achieve the effects of good reproducibility, accurate evaluation and simple pretreatment.

Active Publication Date: 2017-10-24
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current "Chinese Pharmacopoeia" has relatively simple quality control methods for coltsfoot flowers, only taking the content of coltspara as a single index f

Method used

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  • Construction method and applications of tussilago farfara l liquid phase fingerprint
  • Construction method and applications of tussilago farfara l liquid phase fingerprint
  • Construction method and applications of tussilago farfara l liquid phase fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The determination of embodiment 1 chromatographic conditions and the designation of characteristic peaks

[0032] 1. Selection of mobile phase

[0033] Five kinds of mobile phase systems were selected in the experiment: methanol-water, acetonitrile-water, acetonitrile-0.03% acetic acid water, acetonitrile-0.03% formic acid water, 0.03% formic acid acetonitrile-0.03% formic acid water for HPLC of Coltsfoot flower Determination. as attached figure 1 As shown, seven higher chromatographic peaks can be seen in both the methanol-water system and the acetonitrile-water system, but the chromatographic peaks appear later in the methanol-water system, and the number of characteristic chromatographic peaks in the acetonitrile-water system is less ; The baseline drift is more serious in the acetonitrile-0.03% acetic acid water system and the acetonitrile-0.03% formic acid water system, and the resolution of each peak in the acetonitrile-0.03% acetic acid water system is low and ...

Embodiment 2

[0046] Embodiment 2 Fingerprint Atlas Methodological Investigation

[0047] The need testing solution of following experiment all adopts following method preparation:

[0048] Extract 1.0 g of winter flower powder, add 20 mL of 75% methanol, ultrasonically extract for 1 hour, make up the lost weight with 75% methanol, and pass the solution through a 0.22 μm microporous membrane to obtain the test solution.

[0049] Precision experiment:

[0050] Extracting winter flower medicinal material (SX-9), continuous sample injection 6 times, using No. 12 peak as reference peak, record the relative retention time and relative peak area of ​​other characteristic peaks, the result shows that the RSD of the relative retention time of each chromatographic peak is in 0.02% to 0.66%; the RSD of the relative peak area is 0.60% to 2.05%, indicating that the test solution of Coltsfoot flower is analyzed by high performance liquid chromatography, and the precision of the whole detection system i...

Embodiment 3

[0055] Example 3 Comparison of Coltsfoot Flower Liquid Fingerprints from Different Places of Origin

[0056] 26 batches of coltsfoot medicinal materials from different origins (Table 1) were determined using the liquid fingerprint conditions of coltsfoot flower established above.

[0057] Extract 1.0 g of winter flower powder, add 20 mL of 75% methanol, ultrasonically extract for 1 h, make up for the lost weight with 75% methanol, pass the solution through a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the test solution. The chromatographic column Venusil MP (250mm×4.6mm, 5μm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0058] UV detection wavelength is 254nm / 220nm switching detection; column temperature is 25°C; injection volume is 10μL; flow rate is 1mL / min; gradient elution conditions are 0-2min, 5% A phase; %A phase; 7-35min, 15%-30%A phase; 35-38min, 30%-55%A phase; 38-45...

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Abstract

The invention discloses a construction method and applications of a tussilago farfara l HPLC fingerprint, and relates to the technical field of construction of fingerprints. The construction method comprises: (1) extracting tussilago farfara l by using methanol, and filtering with a filtration membrane to obtain a test solution; (2) detecting with a high pressure liquid chromatograph to obtain a chemical fingerprint; and (3) analyzing the chemical fingerprint, wherein the results determine that the tussilago farfara l liquid phase fingerprint contains 14 characteristic peaks. According to the present invention, the construction method has advantages of simple and convenient sample pretreatment and good reproducibility, can accurately reflect the whole quality characteristics of the herb tussilago farfara l, and is used for the quality control of the herb tussilago farfara l.

Description

technical field [0001] The invention relates to column chromatography, in particular to a method for constructing a liquid phase fingerprint of coltsfoot flower and its application in quality detection. Background technique [0002] Coltsfoot flower is a traditional Chinese medicinal material in my country. It has the effects of moistening the lungs, relieving cough and reducing phlegm. It contains phenylpropanoids, terpenes, flavonoids, steroids, alkaloids, volatile oils and other ingredients. The current "Chinese Pharmacopoeia" has a relatively simple quality control method for coltsfoot flowers, which only uses the content of coltspara as a single index for quality evaluation of medicinal materials, and the determination of a single index is difficult to truly reflect the quality of medicinal materials as a whole. [0003] As an effective quality control method for multi-component chemical composition samples, fingerprint technology can reflect the integrity and character...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 李震宇贾岩邢婕秦雪梅
Owner SHANXI UNIV
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