Immunochromatographic test strip for detecting cardiac infarction and heart failure and preparation method thereof

Abstract

The invention discloses an immunochromatographic test strip for detecting cardiac infarction and heart failure and a preparation method thereof, and is intended to provide an immunochromatographic test strip that provides detection results accurately determinable and causes few cross reactions. The immunochromatographic test strip comprises a bottom board; a sample pad, a binding pad, a reaction film and an absorbing pad are linked to the bottom board; CRP (C-reactive protein) antibody and chicken IgY labeled by 300 nm immunofluorescence spheres are sprayed to the binding pad, and NT-proBNP antibody labeled by 200 nm immunofluorescence spheres is also sprayed thereto; the reaction film is parallelly provided with a C quality control line, a T1 detection line and a T2 detection line; the C quality control line is coated with goat anti-chicken IgY; the T1 detection line is coated with antigen CRP protein; the T2 detection line is coated with anti-NT-proBNP antibody.

Description

technical field [0001] The invention relates to a combined detection test paper, specifically, an immunochromatographic test paper for detecting myocardial infarction and heart failure, and also relates to a preparation method of the detection test paper. Background technique [0002] C-reactive protein is one of the acute phase reaction proteins. Studies have found that the serum of patients with acute infection can undergo a precipitation reaction with the C polysaccharide on the cell wall of Streptococcus pneumoniae. It was later confirmed that a protein was involved in the reaction, so it is called C-reactive protein. . The gene is located on chromosome 1 q23, and its sequence is highly conserved. C-reactive protein is one of the members of the penetratingin family. It is composed of 5 identical subunits combined in a non-covalent bond to form a symmetrical ring-shaped pentasphere surrounded by a Pore ​​structure, the concave surface contains ligand binding sites, each ...

AI Technical Summary

Problems solved by technology

[0007] However, the single detection results of hs-CRP and NT-proBNP detection reagents are not clear, and they can only play a guiding role in clinical practice;

[0008] On the one hand, hs-CRP and NT-proBNP detection reagents alone waste samples, and on the other hand, adding multiple samples may lead to irrelevant results

[0009] Combining two different items on one test card, on the one hand, there is interference between the two items, and there is usually a cross-reaction, and on the other hand, it is relatively difficult to interpret the results of the two items.

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